橄榄油中角鲨烯的提取与检测.pdf

食品与效酵科拉
Food and Fermentation Technology
第49卷(第5期)Vol.49,M.5
橄榄油中角鲨烯的提取与检测
龚丽,常伟,黄湛,卢靖,赵辉平,王猛,车振明
(西华大学生物工程学院,四川成都610039
摘要:本文建立了橄榄油中角鲨媂的提取及检測方法。研究形成以橄欖油为原料,利用酯化分离得到橄榄油
的不可皂化物经柱层桁吸附分离純化得到角鲨烯,并对该角鲨烯进行HmPC分析。結果显示:该橄榄油中角鲨烯
的含量为569.2lmg/10g,且提取所得角鲨烯純度为84.33%,对高鈍度角鲨烯的生产有着重要指导作用;该HPIC
检测方法在0.2-.8mg/mlI.范国内线性关系良好,相关系数为0.970,相对标准偏差为1.04%,方法回收率較高,定
量准确,灵敏度高,可用于角鲨烯的定量分析。
关键词:橄榄油;角鲨烯;提取;检測;高效液相色谱法
中图分类号:TS201.2TS207.3
文献标识码:A
文章编号:1674-506X(2013)05-0072-0003
The Extraction and Detection of Squalene in Olive Oil
GONG Li, CHANG Wei, HUANG Zhan, LU Jing, ZHAO Hui-ping, WANG Meng, CHE Zhen-ming
(School of Bioengineering xihua verily, hengtu 610039, China)
Abstract In this paper, an efficient extraction and detection method of squalene derived from olive oil was
proposed. After the esterification reaction from the olive oil, the obtained unsaponifiable substance was further
purified by the chromatography column to get squalane, which content was determined by IIPLC. Results showed
that the squalene content of this olive oil reached 56921m/100g, and the purity was 84.33. IPLC detection
method showed a good linear relationship in the range of 0.2-1.8m /ml, and the correlation coefficient was 0
9970. and relative standard deviation reached 1.04 Additionally. it can be suggested that the method exhibite
high accuracy, sensitivity and recovery, which could be applied to quantitative analysis of squalene
Key words: olive oil squalane: exiraction: delection; HPI.C
doi:10.3969/-issn.1674-.506X.201.3.05-018
角鲨烯是一种脂质不皂化物,最初从角鲨鱼肝少数含量较多,如橄榄油含有角鲨烯150-700mg/
脏中发现的一种多不饱和烯烃(三十碳六烯),由6100g。
个异戊二烯组成的三萜,为淡黄色不溶于水的油状
受生态环境的影响,动物资源越來越稀缺,从植
液体。角鲨烯具有提高体内超氧化物岐化酶(SOD)物油中提取角鲨烯已成必然趋势。目前已有多种成
活性、増强机体免疫能力、改善性功能、抗衰老、抗疲熱的提取技术,例如陈全斌等利用溶剂提取法从
劳、抗肿瘤等多种生理功能,是一种无毒性的具有防罗汉果种仁中分离提取角鲨烯并制得角鲨烯精品;
病治病作用的海洋生物活性物质。角鲨烯在自然界V' azquez等应用逆流超临界CO2萃取橄榄油脱臭
分布较广,深海鲨鱼肝中的含量较高;在植物中分布馏出物中的角鲨烯;lu等应用高效逆流色谱分离
广但含量不高,多低于植物油中不皂化物的5%,仅纯化从微藻中制备角鲨烯; Setiyo Gunawan等·采用
收稿日期:2013-09-05
作者简介:龚丽(1989-),女,硕士研究生研究方向:食品营养与安全
通讯作者:车振明(1960-).男,教擾,硕士生导师。研究方向:食品营养与安全。
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