食源性致病菌免疫学检测方法研究进展.pdf

第6卷第9期
食品安全质量检测学报
Vol 6 No 9
2015年9月
Journal of Food Salcly and Quality
15
食源性致病菌免疫学检测方法硏究进展
章钢刚,赖卫华
南昌大学食品科学与技术国家重点实验室,南昌30047)
摘要:食源性致病菌广泛存在,因其能引起人的疾病甚至死亡,而越来越受世界各国的关注。食源性致病菌
传统检測方法为培养法,其费时耗力,不适用于快速检测。本文介绍的免疫学方法具有方便、快速等优点,因
而越来越被广泛应用。免疫学检测方法主要有酶联免疫吸附法、免疫层析法、免疫磁珠分离技术、化学发光
免疫分析法和乳胶凝集法等。本文着重对这些方法的原理及其应用进行综述.闻述其优缺点,同时对免疫学检
测方法未来的发展趋势进行了展望。
关键词:食源性致病菌;免疫学方法:快速检测
Research progress of immunological detection methods of
food borne pathogen
ZHANG Gang-gang, LAT Wei-hua
National Key Laboratory of lood Science and Technology in Nanchang University, Nanchang 330047, China)
ARSTRAC T: foodborne pathogen widely exists and has drawn more and more attention as it can cause human
discasc even to death. Traditional detection mcthod of foodborne pathogens Is very timc-consuming and canno
meel the need of rapid detection. Immunological detection methods are convenient and rapid, includi
envyme-linked immunosorbent assay, immunochromatography, immunomagnetic beads separation techniques
chemiluminescence immunoassay and latex agglutination assays. In this paper, the principle, application,
advantages, and disadvantages of thesc mcthods were revicwed. The future devclopmcnt trend of the
immunological detection method was prospected
KEY WORDS: foodborne pathogens; immunological method; rapid detection
越来越多。
食源性致病菌检测方法一般是传统培养法,前增菌
引发食品安全问题的因素有很多,比如食品中的毒
需要8~18h,增菌需要18-241h,平板分离需要1848h
素、非法添加剂、食源性致病苗等。食源性致病苗是指能生化试验需要3648b,还要进行复杂的血清学鉴定,耗时
够在食品基质中生存或者生长、井通过人摄食而进入人体耗力,很难做到高通量快速检測,亟需建立快速便捷的检
导致病变的微生物。由致病茵引起的食源性疾病是食品安测方法。
全最大的威胁,其引起的食品安全事件最近国内的报道也
免疫学检测方法一般是应用抗体或抗原与相应的抗
基金项目:南昌大学食品科学与技术国家重点实验室自由探索课题(SKLF-ZZB-201307)、南昌市科学技术局专项(2012-CYH-DWSP-001)
Fund: Supported by the Frec Explorc Issuc of State Key Laboratory of Food Scicncc and Tcchnology of Nanchang Univcrsity
(SK1. -/. /13-201307), Science and Technology project of Nanchang (2012-YII-1)W-SP-001
通讯作者:赖卫华,博士,教授,主要研究方向为食品质量安全。T-mail:lalklolaiwhの163.com
Corresponding author: LAI Wci-hua, Ih. D, Irofcssor, National Kcy Laboratory of Food Scicncc and Tcchnology in Nanchang Univcrsity,
No.235.NaniingEastRoad,Nanchan330047.China.e-ma1l:talktolaiwh(c163.com