液相色谱-四级杆飞行时间质谱筛选代谢对羟基苯乙酮的芽胞杆菌.pdf

第8卷第6期
食品安全质量检测学报
Vol 8 No 6
2017年6月
Journal of Food Safety and Quality
Jun.,2017
液相色谱-四级杆飞行时间质谱筛选代谢对羟基苯
乙酮的芽胞杆菌
陈峥,刘波',朱育菁”,刘晓港,潘志针
(福建省农业科学院农业生物资源研究所,福州35000
摘要:目的使用液相色谱-四级杄飞行时间质谱筛选代谢对羟基乙酮的芽胞杆菌。方法以20种不同芽
胞杆菌为硏究材料,采用液相色谱-四级杆飞行时间质谱( liquid chromatography/ quadrupole time-of- flight mass
spectrometry, LC-QTOF-MS)技术,通过分子特征提取和谱库检索,获得总体代谢物信息。结果筛选出1株
能够代谢对羟基苯乙酮的芽胞杆菌,为福氏芽胞杆菌FJAT-10032,测试结果中,该化合物精确分子量为
136.0522,与 Metlin谱库检索得分为90.97,占菌株FJAT-10032总代谢物相对含量为1.39%。结论福氏芽
胞杆菌能够产生对羟基乙酮,本研究为该菌的开发和利用提供理论依据。
关键词:液相色谱-四级杆飞行时间质谱;福氏芽胞杆菌;对羟基苯乙嗣
Screening of Bacillus strains producing 4-hydroxyacetophenone by liquid
chromatography/quadrupole time-of-flight mass spectrometry
CHEN Zheng, LIU Bo, ZHU Yu-jing, LIU Xiao-gang, PAN Zhi-zhen
(gricultural Bio-resources Research institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China)
ABSTRACT: Objective To screen the Bacillus strains producing 4-hydroxyacetophenone by liquid
chromatography/quadrupole time-of-flight mass spectrometry(lc-qtof-ms) Methods Extracellular metabolites
of 20 Bacillus strains were analyzed by LC-QTOF-MS. The information of total metabolites was obtained by
molecular feature extraction and spectral library search. Results The results showed that only one Bacillus strain
Bacillus fordii FJAT-10032 that producing 4-hydroxyacetophenone. The exact mass of the compound was 136.0522
The relative content of 4-hydroxyacetophenone was 1.39% with match score of 90.97. Conclusion This study
rovides the theory basis for the development and utilization of Bacillus fordii FJAT-10032, which can produce
4-hydroxyacetophenone
KEY WORDS: liquid chromatography/quadrupole time-of-flight mass spectrometry; Bacillus ford
4-hydroxyacetophenone
基金项目:国家自然基金项目(31370059)、福建省公益性专项(2015R1018-1)
Fund: Supported by the National Natural Science Foundation of China(3 1370059)and ommonweal Project of Fujian Province(2015r1018-11
通讯作者:刘波,研究员,主要研究方向为徴生物生物技术与农业生物药物。E-mail:fzliubo(@163.com
朱育菁,博土,研究员,主要研究方向为农业生物药物与生物防治。E-mail: zyjingtz(163,COm
+ Corresponding author: LIU Bo, Professor, Fujian Academy of Agricultural Sciences, Fujian 350003, China. E-mail: liubo@ 163. om
ZHU Yu-jing, Ph. D, Professor, Agricultural Bio-resources Institute, Fujian Academy of Agricultural Sciences, Fujian
350003,China.E-mail:zyjingfz(@163.com
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