烯啶虫胺在棉花和土壤环境中的残留及消解动态研究.pdf

第6卷第3期
食品安全质量检测学报
Vol 6 No. 3
2015年3月
Journal of Food Salcly and Quality
Mar..20)15
烯啶虫胺在棉花和士壤环境中的残留及
消解动态研究
李增梅,邓立刚,赵善仓,张树秋,郭长英,梁京芸,董燕婕
(山东省农业科学院农业质量标准与检测技术研究所,山东省食品质量与安全检測技术重点实验室,济南250100
摘要:目的建立操作简单、回收率较高的浄化方法.并应用该净化方法,建立烯啶虫胺的高效液相色谱
(HPLC)检測方法,研究其在棉叶和土壤中的代谢残留及最终残留。方法样品经甲醇/水(v:v,60:4の)提取.,PCX
固相萃取柱净化后,经 Waters Atlantis Ci%(3.6mm×250mm,5um)色谱柱分离。结果棉叶、棉籽、土壤中烯
啶虫胺的最低检出浓度OQ均为0.01mg/kg:在0.02~10gmL范围内烯啶虫胺的峰面积与质量浓度的线性
关系良好,R=0.999。、在棉叶、棉籽和士壤样品中三个不同浓度水平的添加回收率在71.5%~94.5%之间、RSD
为2.9906.5%。代谢结果表明,烯啶虫胺在棉叶和土壤中的消解半衰期分别为山东2.8-5.1天,浙江2.1-4.3天
最终残留结果表明、烯啶虫胺在棉籽和土壤中的最终残留量均未检出。结论该方法简便、准确可靠,适用于
棉叶和土壤中烯啶虫胺含量測定,并且烯啶虫胺属于昜降解农药。
关键词:液相色谱法;烯啶虫胺;棉花;土壤;残留
Residue decline study of nitenpyram in cotton and soil
LI Zeng-mei, DENG Li-gang, ZHAO Shan-cang, ZHANG Shu-qiu
GUO Chang-ying, LIANG Jing-yun, DONG Yan-jic
(Key Laboratory of Yest echnolog on Hood Ouality and Safery of Shandong Province, Ainstitute of Agricu/tura Ouality
Stand d esting ecm Eeac handong Academy Agicuiasciences, inom 250100, him)
ABSTRACT: Objective To stablish a method for the dctermination of nitenpyram in cotton leaves and soil
by high performancc liquid chromatography (PLC). Degradation of nitenpyramin cotton and soil using this
TPI C method was studied. Methods Samples were extracted by methanol/water(60: 40, v: 1), and then
purified by PC cxtraction column. The analysis were scparated on a Waters Atlantis C18(3.6 mmx250 Im,
um)and detected by IIPLC. Results This method provided a high Iinearity of the working curve for
calibration as well as repeatability and reproducibility. The limits of quantification (1.OQ)were 0.01 mg/kg in
cotton and soll. The corrclation coefficient of the working curve for calibration was 0.9999 for the nitenpyram
in the concentration range from 0.02 to 10 ugml,. The recoveries of nitenpyram in cotton, secd and soil wcre
simple and accurate for the determination of nitenpyram. Nitenpyram was an easily degradable insecticide
715-94.5, and Swee 2. -. The results showed the half-lives of nitenpyram were 2.8-5.1 d al
cotton, and 2. 14.3 d in soil. The final residues of nitenpyram were undetectable. Conclusion The metl
KEY WORDS: high performance liquid chromatography, nitenpyram, collon, soil, residues
通讯作者:邓立刚,副研究员,主要研究方向为食品质量与安全检测技术。F- mail: deny ligang(163.c0m
Corresponding author DENG Li-gang, Rcscarch Associate, Institutc of Agricultural Quality Standards and Testing Tcchnology Rcscarch
ShandongAcademyofAgriculturalScicnccs,jinan250100,China.E-mail:dcngligang(@163.com