食品病原微生物快速检测技术研究进展.pdf

第6卷第1期
食品安全质量检测学报
Vol 6 No
2015年1月
Journal of Food Salcly and Quality
Jan..2015
食品病原微生物快速检测技术研究进展
杨春光,王宏伟,彭心婷2,苏明明,徐静,李莉,孙兴权,曹际娟1
(1.辽宁出入境检验检疫局.大连116001:2.塔域出入境检验裣疫局.塔城834700
摘要:随着现在食品工业的快速发展,食品安全越来越受到各国的重视。影响食品安全的因素很多,其中食
品中的病原微生物是影响食品安全的主要因素之一。为了对食品安全进行有效、快速的监測,控制食品加工中
的病原微生物、研究和建立食品病原快速检测方法对于食品质量控制和监管及人们健康也就越来越重要。食源
性致病菌的传统检测方法繁琐复杂、周期较长,因而快速、简便、特异的检测方法成为研究的热点。快速检测
方法的应用范围非常广泛,井且比传统的检测方法更加敏感。本文主要从分子生物学技术、免疫技术、代谢技
术、生物传感器技术等方面介绍了目前国内外用于食品微生物检测的先进技术,并对这些在当前较为先进的主
流的快速检测技术进行总结分析,为今后进一步研究和开发新检测技术提供一些参考
关键词:快速检测;分子生物学技术;免疫技术;代谢技术;生物传感器
Research advances for fast detection of food borne pathogenic bacteria
YANG Chun-guang, WANG Hong-wci, PENG Xin-ting, SU Ming-ming, XU Jing
LI Li, SUN Xing-quan, CAO Ji-juan
(1. Liaoning Entry-exit inspection and uarantine Bureau, Dalian 116001, China
2. Tacheng Fntry-fxit inspection and Quarantine Bureau, lacheng 834700, China)
ABSTRAC T Now with the rapid development of food industry, food safety is concerned all over the world
Many factors affect the food safety, among which pathogenic microbe is onc of the most primary factor. In
order to effectively and efficiently monitor the food salely, il is more and more imporlant for food qu
control and supervision to control the pathogenic microorganism in food processing, and establish the method
for rapid detection of food pathogen. Traditional methods for detection of food borne pathogens are quito
complex, and cost much time, so more researches focus on the quick, convenient and dilterenlial methods. The
scope of application of rapid detection method is very extensive, and more sensitive than the traditiona
detection method. This article introduced several advanced fast detection technology of food pathogenic
bacteria, including molecular biology technology, immunity technology, metabolism technology and biosensor
technology, and the rapid detection lechnology of [hose more advanced analysis and summarize the current
mainstream, aiming to provide a reference for the further study
KEY WORDS: quick detection molecular biotechnology, Immunity technology; metabolism tcchnology,
biosensor technolo
通讯作者:曹际娟、研究员,主要研究方向为食品安全检测。T-mail:cij090c9163.c0m
Corresponding author; CAO Ji-juan, Irofcssor, Tcchnical Ccntcr of Li
Entry-exit Inspcction &Quarantinc Burcau, No 60, Changjian
East Road, Zhongshan District, Dalian 116001, China. E-mail: c1j0909(163. com