高效液相色谱法测定松针中莽草酸的含量.pdf

第22卷第7期
化学研究与应用
Vol, 22 No. 7
2010年7月
Chemical Research and Application
July, 2010
文章编号:1004-1656(2010)07-0943-05
高效液相色谱法测定松针中莽草酸的含量
谢济运2,陈小鹏',粟本超,陈芳,李志荣
(1.广西大学化学化工学院,广西南宁530004;
2.柳州师范高等专科学校,广西柳州545004)
关键词:高效液相色谱法;松针;莽草酸
中图分类号:0657.7文献标识码:A
Determination of the content of shikimic acid in pine needles
by high performance liquid chromatography
XIE Ji-yun", CHEN Xiao-peng " SU Ben-chao', CHEN Fang IT Zhi-rong
(1. School of Chemistry and Chemical Engineering, Guang xi University, Nanning53000, China;
2. Department of Chemistry and Life Science, Liuzhou Normal College, Liuzhou 545004, China
Abstract: The content of shikimic acid in Pinus elliottii needles were determined by high performance liquid chromatography
(HPLC), samples extracted with petroleum ether defatted after distillation of water, then extraction and purification by using n
butanol and 5% NAOH, respectively, and finally carry out separation and detection by HPLC. The mobile phase was Hexane 2%
H PO (90: 10) the flowing velocity was 1 ml/min Alltima NH(5 wm, 4. 6 mm x 150 mm)column separation, test wavelength
was 213 nm. Shikimic acid in 0.3-3. 3 w /WL range of linear relationship, comelation coefficient of 0.9992 Shikimic acid to be
the average recovery was 98. 5%. RSD=0. 85%(n=5). Pinus elliottii needles of dry extract on of shikimic acid in the average
rate of 1. 32@0
Key words: HPLC: pine needles; shikimic acid
松针为我国传统中药,《本草纲目》曰:松针,松针中含有溶解人体内老化物,调整和促进身体
味苦温、无毒,久服令人不老,轻身益气,主治风湿组织机能,增进人体健康的物质生长,并能有增强
疮,生毛发,安五脏,守中,不优,延年。历代均免疫力和提高抵抗能力的作用,还被广泛用于治
入药用,民间用于治疗神经衰弱、维生素丙缺乏疗风湿病、心脑血管疾病、糖尿病和肥胖症等
症、营养性水脚、风牙肿痛、阴囊湿痒、腰痛、跌打症?;松针含有莽草酸成分,现代药理学研究还
损伤和预防钩虫病]。现代临床医学上常用以治证明,莽草酸具有较强的镇痛和抑制血小板聚集
疗慢性气管炎、预防流脑和感冒。国外学者认为作用”),其价值日益受到重视。特别是2005年
收稿日期:2009-11-17;修回日期:201002-27
基金项目:国家自然科学基金资助项目(30560119);广西自然科学基金资助项目(0991030);广西研究生教育创新计划项目
(2008105930817M46);广西教育厅科学研究项目([2006126);广西大学科研基金项目(X081020):广西青年基金项目(0832008);广西教
育厅科学研究项目(200708LX180)
联系人简介:陈小鹏(1954-)男,教授,从事精细化工与林产化工研究。Email:lilm@gu.edu.cn