嗜水气单胞菌、爱德华氏菌灭活菌苗免疫保护及药物治疗试验.pdf

广西农业科学2010,41(3):266-269
Guangxi Agricultural Sciences
嗜水气单胞菌、爱徳华氏菌灭活菌苗免疫保护
及药物治疗试验
邓显文,谢芝勋,刘加波,谢志勤,谢丽基,庞耀珊
(广西兽医研究所,南宁530001)
摘要:嗜水气单胞菌、爱德华氏菌培养物经甲醛灭活后,制成灭活油乳剂苗、灭活菌苗、饵料吸附型疫苗,免疫罗
非鱼2周后,分别以嗜水气单胞菌、爱德华氏菌活菌液攻毒,结果显示,注射型疫苗均优于口服型,其中嗜水气单胞菌
以腹腔注射0.1mL嗜水气单胞菌灭活菌苗+0.3mL生理盐水的效果最佳,免疫保护率为85.0%;而爱德华氏菌以腹腔注
射0.4m.灭活油乳剂苗效果最佳,免疫保护率为90.0%。两者的药物治疗试验结果显示,以氟苯尼考、恩诺沙星、环肉沙
星、氟哌酸治疗罗非鱼嗜水气单胞菌病、爱德华氏菌病均有一定效果,对减少发病率及死亡率、控制病情其有较好作
用,其中以氯苯尼考的疗效最佳,其用量为20 mg/kg H1O。
关键词:罗非鱼;嗜水气单胞菌;爱德华氏菌;灭活菌苗;免疫保护;药物治疗
中图分类号:S965.12
文献标识码:A
文章编号:1002-8161(2010)03-026604
Protective immunity in tilapia against inactivated bacterin of
Aeromonas hydrophila and Edwardsiella and controlling effects
of antibacterial medicines
DENG Xian-wen, XIE Zhi-xun, LU Jia-bo, XE Zhi-qin, XIE Ii-j, PANG Yao-shan
(Guangxi Veterinary Research Institute, Nanning 530001, China)
Absract: The colonies of Aeromonas phila and Edwardiella trains were cultured on nutrition broth medium
for 4 h at 8 the strain luon was o eed and inacti ed with fml eh e ton for 2 days for the preparation
of inactivated oil-adjuvant vaccine activaed bacterin and bai-ab ed vaccine. The tilapia was immunized with three
inactivated bacterin for two weeks and injected with live strain solution of Aeromonas hydrophila and Edwardsiella. The re-
suls showed that the immune effects of inactivated oil-adjuvant vaccine and inactivated bacterin were beer than that of
bait -absorbed vaccine. Out of all treatmen, injecting .I ml inactiated bacterin and .3 ml nomal saline injected o
Aeromonas hydrophila and 0. 4m inactived il-adjuvan ac ne Edwad ella were found to be the best at85.0% and
90.0 of vaccine pret a eie Te antibiotics erapy esting showed that florfenicol, nrofloxaci
ciprofloxacin and nofoxaci d therapeuti ef s n ilap a infected with Aeromonas hydrophila and Edwardsiella
for decreasing the incidence rae and death rate and among them 20 mgkg H 0 florfenicol was found to be the best in
controlling these two pathogens
Key words: tilapia; Aeromonas hydrophila: Edwardsiella; inactivated vaccine; protective immunity; medicine therapy
嗜水气单胞菌( Aeromonas hydrophila)隶属弧迟钝爱德华氏菌也是一种重要的人畜共患病的病
菌科( Vibrionaceae)、气单胞菌属( Aeromonas),可引原菌。嗜水气单胞菌、爱德华氏菌均能引起多种
起鱼类致死性出血性败血症,是鱼类疾病的主鱼类感染发病,尤其是淡水鱼,因此给淡水养殖业
要病原,也是一种典型的人一兽一鱼共患病病原造成巨大经济损失。目前,防治鱼病的主要方法是
菌?。鱼类爱德华氏菌属( Edwandsiellatarda)中主依靠抗生素等化学药物,但化学药物的使用容易对
要有迟钝爱德华氏菌(E. tarda)、鲇鱼爱德华氏菌水体等环境资源造成污染,因此,有必要加强鱼类
( A. ictal)和保科爱德华氏菌( E hoshinae),其中,疫苗的研制及特效药物的筛选。本研究以罗非鱼为
收稿日期:2009-11-10
基金项目:广西科技攻关项目(桂科攻0428001-2
作者简介:邓显文(1963-),男,广西贺州人,副研究员,主要从事动物疫病分子生物学研究工作。*为通信作者,E-mail
xiezhixunt@126.com。
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