邻苯二甲酸二乙酯对蟾蜍蝌蚪红细胞微核的影响.pdf

第27卷第1期
山东建筑大学学报
ol.27
012年2月
JOURNAL OF SHANDONG JIANZHU UNIVERSITY
Feb
文章编号:1673-7644(2012)01-0025-03
邻苯二甲酸二乙酯对蟾蜍蝌蚪红细胞微核的影响
张维建',石兴玲2,张超
1.山东建筑大学市政与环境工程学院,山东济南250101;2.山东建筑大学外语学院,山东济南250101)
摘要:为探讨邻苯二甲酸二乙酯对蟾蜍蝌蚪的毒害作用,采用不同浓度邻苯二甲酸二乙酯溶液对蟾蜍蝌蚪进行
处理(终含量0,1.6,3.2,6.5,13,26ugy/m)检測蝌蚪红细胞微核率和核异常细胞率。结果显示伴随邻苯二甲
酸二乙酯浓度的升高,蝌蚪红细胞微核率及核异常细胞率升高,邻苯二甲酸二乙酯浓度为13pug/mL(与对照组
相比P<0.01)差异显著。相关分析显示邻苯二甲酸二乙酯与蝌蚪红细胞微核率及核异常细胞率高度相关并
存在剂量一效应关系。邻萃二甲酸二乙酯对蟾蜍蝌蚪有明显的遗传毒性。
关键词:邻苯二甲酸二乙酯;蟾蜍蝌蚪;红细胞;微核率
中图分类号;X171.5
文献标识码:A
Effects of diethyl phthalate on tadpole erythrocyte micronucleus
ZHANG Wei-jian, SHI Xing-ling, ZHANG Cha
(1. School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan 250101, China
2. School of Foreign Languages, Shandong Jianzhu University, Jinan 250101, China)
Abstract: In order to explore toxicity induced by diethyl phthalate, toad tadpoles are exposed to differ-
ent concentration of diethyl phthalate(the termina concentration are 0, 1. 6, 3.2, 6.5, 13, 26ug/ML)
to investigate the effects of diethyl phthalate on micronucleus(MNC) rate and nuclear abnormal cells
Results show that MNC and nuclear abnormal cells of toad tadpoles significantly increase with the in-
crease of concentrat on of diethyl phthalate(compared with the control group P<0. 01), and the
difference is significant when concentration of diethyl phthalate is at 13 /ml. Corrlation anal ysis
shows that remarkable corrlation is found between the diethyl phthalate, MNC and nuclear abnormal
cells and has a significant dose-effect relation. These results suggest that diethyl phthalate could in
duce the genotoxic effects on the toad tadpoles
Key words: phthalate; toad tadpoles; RBC; micronucleus
出最多的有机污染物之
有关邻苯二甲酸酯
类(PAES)物质的遗传毒性研究,国内见有胡晓晴
陈如等的报道,国外见有M. Mariko,H.K. Mutsu
邻苯二甲酸二乙酯是生产聚氯乙烯的原料。伴ko的报道2の,但仅限于邻苯二甲酸二丁酯和邻苯
随我国聚氯乙稀产业的发展,邻苯二甲酸二乙酯造二甲酸二甲酯等对鱼类、藻类的毒性研究,对蟾蜍
成的水质污染日趋严重。该物质已成为水环境中检蝌蚪红细胞微核的影响未见报道。本文探讨了邻苯
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作者简介:张维建(1958-),女,山东昌乐人,教授,从事环境生态毒理学研究,E-mail.2みhangwj@@sdjzuedu.cn