应用酶联免疫分析方法检测镇江市内水域水样和底泥中的邻苯二甲酸二乙酯.pdf

2016,35(11):2237-2244
农业环境科学学报
2016年11月
Journal of Agro-environment Science
曾昆,周军,邵杰,等.应用酶联免疫分析方法檢测镇江市内水域水样和底泥中的邻苯二甲酸二乙酯り.农业环境科学学报,2016,35(11
2237-2244.
ZENG Kun, ZHOU Jun, SHAO Jie, et al. Development of a enzyme-linked immunosor bent assay for diethyl phthalate analysis in water and sediment samples
rom rivers in Zhe jiang City[] ourn of A r-enpionment Science, 2016, 35(11): 2237-2244
应用酶联免疫分析方法检测镇江市内水域水样和
底泥中的邻苯二甲酸二乙酯
曾昆…,周军1,邵杰1,张祯"
(1.江苏大学环境与安全工程学院,江苏镇江212013;2.江苏大学环境生态研究所,江苏镇江.212013)
摘要:应用邻苯二甲酸二乙脂( Diethyl phthalate,DEP)抗体,建立了间接竞争性 ELSA方法,并对反应条件进行优化,进面测定了
镇江市内水域中DEP含量。结果配示优化后的 ELISA方法檢測范固为20-320ng·mL1,最低檢测限为8.2ng-mL方法特异性高,
与其他邻苯二甲酸酯类似物几乎无交又反应。 ELISA方法批内差在3.38%~9.09%,批间差在3.76%~12.78%。檢测方法檢测水样和底
泥样本中DEP含量,添加回收率在90.46%-12275%。镇江市内古运河和运粮河水样和底泥样本中DEP的检出率分别为62.5%和
875%,其中水样中DEP含量最高可为6.61ng·mL1,底泥样不中DEP含量最高为86.9ngg
关键词:朝化剂;DEP;抗体
中图分类号:X78文献标志码:A文章编号:1672-2043(2016)11-2237-08doi:10.11654aes.2016-0303
Development of a enzyme-linked immunosorbent assay for diethyl phthalate analysis in water and sediment
samples from rivers in Zhenjiang City
ZENG Kun 2, ZHOU Jun. SHAO Jiel. ZHANG Zhen
(1. College of Environmental and Safety Eng ineering, Jiangsu niversity Zhenjiang 12013, hina nsti ute of Environment and Ecology
Jiangsu University, Zhenjiang 212013, China)
Abstract: Based on a polyclonal antibody aretin diethyl phthalate(DEP a sensitive indirect competitive enzyme-linked immunosorbent
assay( ELISA)was proposed and several parameters were investigated. Under the optimized conditions, the developed method showed high
sensitivity to DEP(he limit of det ion 8.n ml ) and high selectivity witcross-eactiities es an4% even structurally r
lated phthalate esters. The linear range was from 20 ngml to 320 ngml The recoveries(90.46%-12275%)and reproducibility(CV of
intra-assay, 3.38%-9 09%; CV of inter-assay, 3.76%-12.78% )were satisfactory. Our proposed ELISA indicated that it had good agreement
with those given by GC-MS, which was applied to detect DEP in waters and sediments from ancient canal and Yunliang River And DEP
was found in most of target areas(the detection rate, 62.5% in waters and 87.5% in sediments ), the concentration Was up to 6.61 ngml
land 86.9 ng. I in water and sediments, respectively
Keywords: plasticizer; DEP; antibody
邻米二.甲酸( Phthalate esters,PAEs)类塑化剂料用于香味剂、化汝品和冷凝剂3。随着工业生产的
广泛应用于塑料及一次性塑料消费品,还可以作为原发展及塑料制品的大量使用,PAEs已经成为全球性
最普遍的一类污染物們。其中邻苯二甲酸二乙酯(Di
收稿日期:2016-03-10
基金项目:国家自然科学基金项目(31502118,21577051);江苏省白然
ethyl phthalate,DEP)是PAEs中的重要成员,近年来
科学基金项日(BK20130507);中国博士后基金项日
被越米越多地用作增塑剂和食品添加剂,在法
(2013M541606)
国回、印度间、西班牙四以及我国广州、台湾門等地区的
作者简介:曾昆(1980一),女,湖北襄阳人、博士,讲师,从事污染物
快速分析研究。E-mail:kj80116@163.cm
水体和底泥中均有检出。研究表明DEP是一类影响
通信作者:张被E-mail: zhz heruka163com
严重的坏境激素,易造成生物体内分泌失调,引起生
万方数括