不同晶相的草酸钙晶体引起肾上皮细胞的毒性差异.pdf

第32卷第5期
无机化学学报
01. 32 No5
2016年5月
CHINESE JOURNAL OF INORGANIC CHEMISTRY
818-826
不同晶相的草酸钙晶体引起腎上皮细胞的毒性差异
孙新园姚秀琼余凯欧阳健明
(暨南大学生物矿化与结石病防治研究所,广州510632)
摘要:采用扫描电子显微镜SEM)、激光共聚焦显微镜SM、流式绍胞仪、电感耦合等离子体发射光谱仪(TCP等方法比较研究
了尺寸约1.2m的一水草酸钙(COM和二水草酸钙COD)晶体对非洲绿猴肾上皮细胞(Vero细胞)的损伤差异。COM和COD均
能引起Ve细胞的细胞活力和超氧化物歧化晦(SOD活力降低、丙二醛(MDA)含量升高以及碘化丙啶(P红染细胞数目增多,且
均呈现浓度依赖性。在相同晶体浓度时,COM的细胞毒性明显比COD强,引起细胞表面表达的粘附分子透明质酸HA)比COD
多,粘附量也显著增加。相比于COM品体,Vero细胞更容易内春COD晶体。COM与COD晶体对肾上皮细胞的毒性差异与晶面
的离子密度以及细胞与晶体间的静电作用等因素密切相关。本文结果将有助于从分子水平上和细胞水平上阐释草酸钙结石的
形成机理。
关键词:一水草酸钙;二水草酸钙;细胞毒性;浓度效应
中图分类号:H69;R329.28
文献标识码:A
文章编号:1001-4861(2016)05-0818-09
DOI:10.11862/CJIC.2016.097
Toxicity Difference of Calcium Oxalate of Different Crystal
Phases on Renal Epithelial Cells
SUN Xin-yuan YAO Xiu-qiong YU Kai OUYANG Jian-ming
(nstitute of Biomineral t on nd thiais Resech nn nie y, Gumg hou 510632, China)
Abstract: Scanning electron microscope (SEM laser confoeal microscope (SM), flow cytometry, inductively
coupled plasma emission spectrometer(CP)methods were used to comparatively study the cell injury differences
on the African green monkey kidney epithelial (Vero)cells by calcium oxalate monohydrate(COM)and d hydrate
COD) crystals with a size about 1.2 wm. Both COM and COD could decrease the cell viability and superoxide
dismutase (SOD )activiy, increase malondialdehyde (MDA)content and the number of red dye cells staining with
propidium iodide (PT)in a dose-dependent manners. Compared to COD crystals, COM crystals induced
significantly higher toxicity under the same crystal concentration, and COM crystals caused more adhesion
molecules hyaluronic acid (A )expression and higher adhesion amount than COD crystals. The ability of the
Vero cells to endocytose COD crystal was stronger than that of COM. The cytotoxicity difference between COM
and COD was closely related to ion density of crystal face and electrostatic interactions between the cell and
crystals. The results in this paper can help to explain the formation mechanism of calcium oxalate stones at the
molecular level and celular level
Key words: calcium oxalate monohydrate; calcium oxalate dihydrate; cytotoxicity; concentration effect
收稿日期:201601-14。收修改稿日期:2016-03-04
家自然科学基金(No.2137107の资助项目。
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