液相色谱串联质谱法测定食品中甘露糖醇、麦芽糖、木糖醇、山梨糖醇.pdf

中国食品加剂
China Food Additives
分标测试
液相色谱串联质谱法沺定食品中甘露糖醇
麦芽糖、木糖醇、山梨糖醇
马书民,王明泰,韩大川,荣会,胡婷婷,康明芹,周晓
(吉林出人境检验检疫局,长眷130062)
摘要:灭然甜味剂是从天然植物中提取出来的,在安全性和营养功能方面远好于人工合成的甜味剂。
本文采用液相色谱串联质譜法测定了食品中露糖醇、麦糖,木糖醇、山梨糖隊的残留量,并采用选择离
子检测进行阳性确证。液体试样用水超声提取,离心定容后,固体和半固体样品采用水溶解定容,再经固相
萃取柱净化,样液经洗脱定容后,供液相色谱一质谱/质谱仪测定,外标法定量。本方法的最低检出限、线性
范和方法回收率分别为:0.10my/kg、0.10~1000.0mg/kg和89.0~94.9%。本研究建立了食品中甘霹糖
醇、麦芽糖、木糖醇、山梨糖醇的检测方法,可以快速、灵敏、一次时测定多个,多类别大然甜味剂及其
附性确证。也为打彼国外技术性贸易壁垒、增我国出口产品在国际巾场竞争力提供了有力的保障。
关键词:食品;H露糖醇;麦爿糖;木糖醇;山梨糖醇;固相萃取;液相色谱串联质增法
中图分类号:TS2073文献标识码
文章编号:1006-2513(2011)05-0206-05
Determination of mannitol, maltose
xylitol, sorbitolin in foods by LC-MS/MS method
MA Shu-min, WANG Ming-tai, HAN Da-chuan, RONG Hui
HU Ting-ting, KANG Ming-qin, ZHOU Xiao
( Jin Lin Entry-exit Inspeetion and Quarantine Bureau, Changchun 130062
Abstract: Natural sweeteners extracted from natural plants, in safety and nutrition function far better than synthetic
sweeteners. In this paper, liquid chromatography-tandem mass spectrometry was used to determine mannitol, malt
ose, xylitol, sorbitolin of the residues in food, using selected ion monitoring for poitive confirmatiom. Liquid sample
is extracted with watcr by the ultrasonic prox ssr, After centrifugation and volume, Solid samples or hemi-solid
ples were dissolved constat volume of water, and then by HB solid phase ex raction cartridges, eluted samle
ion was determined later for the determination and confirmation by LC-MS/MS using extermal standard method. The
detection limits, linear ranges, and recoveries of this method, for mannitol, maltose, xylitol, sorbiolin, are
0.10mg/kg 0. 101000. Omg/kg and 89094.9%. This study established a method for determination of mannitol
maltose, xylit l soritolin in o, can qu ly and sensitive, a il us demint on of many ate rie an
weeteners and its positive categories confirmation. Also is to break the foreign technical barriers to trade, en
hance China export products in the international market competition ability to provide strong uarantee
Key words: foods; mannitol; maltose; xylitol srbitolin; accelarated solven extraction; solid phase extraction
LC-MS/MS
收稿日期:201-07-
基金项目:国家质检总局科研课题(20071143)。
作者筒介:马书民(1978-),男,汉族,工程师,硕士,研究方向为食品检测。
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