解脂耶氏酵母不对称还原苯乙酮合成(R)-苯乙醇.pdf

学w. xitl I IL. c: on
化工进展
CHEMICAL INDUSTRY AND ENGINEERING PROGRESS
2016年第35卷第2期
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研究开发》
解脂耶氏酵母不对称还原苯乙酮合成(-苯乙醇
许芹,王丹,徐晴,李霜
(南京工业大学生物与制药工程学院,江苏南京210009
摘要:为了提高解脂耶氏酵母对苯乙酮的催化效率,以20 mmol/L的苯乙酮和苯乙醇为筛选压力,采用紫外诱变
获得突变株,优化了反应体系参数,如温度、pH值、辅助底物、有机溶剂(如甲苯、己烷、辛烷、DMF、DMSO
等)以及离子液体(1-丁基-3-甲基咪哩六氟磷酸盐,BMIM-PF6)等对催化体系的影响。结果表明,利用解脂耶
氏酵母突变株全细胞催化苯乙酮(20mmoL)合成(R)-苯乙醇,转化率76%,ee.达92%。优化后的反应体系
如下:最适辅助底物为葡萄糖,反应温度30℃,pH=7.0,30%(体积分数)疏水性离子液体BMM-PE6。当利
用解脂耶氏酵母突变株的发酵液为催化体系,向反应体系中添加30%(体积分数)BMM-PP6,以60 mmo/L
苯乙酮为底物,反应36h,(R)-苯乙醇的转化率达95%,e.e.値达97%。解脂耶氏酵母突变株全细胞催化苯乙酮不
对称还原反应,在高底物浓度、高转化率以及高光学纯度等方面具有突出表现。
关键词:解脂耶氏酵母;苯乙酮;(R)苯乙醇;生物催化;醇;优化
中图分类号:Q503
文献标志码:A
文章编号:1000-6613(2016)02-0528-06
DOL:10.16085/jssn.1000-6613.2016.02.028
Asymmetric reduction of acetophenone to (R)phenyl ethanol by Yarrowia
lipolytica
XU Oin, WANG Dan, XU Oing, L/ Shuang
( College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech. University, Nanjing 21009, Jiangsu, China)
Abstract: In order to improve Yarrowia lipolytica's catalytic efficiency for acetophenone, using
20mmol/L acetophenone and 20mmol/L phenyl ethanol as screening pressure, we obtained a mutant
strain through UV mutation. Then we optimized the reaction conditions, such as temperature, PH
co-substrate and organic solvents. The mutant strain Yarrowia lipolytica ACA-DC 50109-H could
reduce 20mmo /L acetophenone to optical pure (R)-phenyl ethanol(76%conversion, 99.2%ee). The
optimal reaction conditions were using glucose as co-substrate, and with +=30C, pl-7.0 and the
addition of 30%o(/)ionic liquid (BMM PF6). When the culture broth of Yarrowia lipolytica
ACA-DC 50109-H was used as the reaction system, with the addition of 30% BMIM-PF6, 60mmol/L
acetophenone could be reduced to (R)-phenyl ethanol (97. 5% conversion, 95% e..) after 36h
Yarrowia lipolytica ACA-DC 50109-H could efficiently catalyze the asymmetric reduction of
a-acetophenone to(R)-phenyl ethanol with high substrate loading, high conversion and ee
Key words: Yarrowia lipolytica; acetophenone;(R)-phenyl ethanol; biocatalysis; enzyme; optimization
含有芳香基的手性醇是许多手性药物合成的关合成反应不但可以省去繁杂的酶分离和纯化步骤,
键手性模块和平台化合物,在手性合成中占有重要收稿日期:20150521:修改稿日期:2015030.
的地位:而前手性芳香酮的不对称催化还原是制备
基金项目:国家重点基础研究发展计划项目(2011CB710800)。
光学活性醇的一个重要方法2?。微生物细胞中含有
第一作者:许芹(199-),女,硕土研究生,主要从事生物催化研究
联系人:李稻,教授,主要从事生物催化与生物转化研究。E-mail
许多具有立体选择性的酶,使用全细胞催化不对称 lishuang(@njtechedu cn