醋酸铅对大鼠睾丸支持细胞活性及抑制素B+mRNA水平的影响.pdf

Journal of Zhengzhou University(Medical Sciences) May 2010 ol. 45 No. 3
411
醋酸铅对大鼠睾丸支持细胞活性及抑制素 B MRNA水平的影响
苏晓东),李春阳2),高慧艳”,赵士光3),陈小玉")
1)郑州大学公共卫生学院卫生毒理学教研室郑州4500012)郑州大学公共卫生学院劳动卫生学教研室郑州4500013)衡
州市疾病预防控制中心衛州324000
通讯作者,女,1957年11月生,硕士,教授,研究方向:生殖与发育毒理,E-mail:chen-xiaoyu@zzu.edu.cn
关键词醋酸铅;擊丸支持细胞;抑制素;大鼠
中图分类号Q492.4
摘要目的:观察醋酸铅对体外培养大鼠睾丸支持细胞活性和抑制素B(INHB)mRNA水平的影响。方法:建
立大鼠睾丸支持细胞体外培养模型,分别给予不同浓度(0、108、10-7、10-°和10-3mol/L)的醋酸铅处理细胞,培
养24h后采用MT法测定细胞活性;同法分组,采用RT-PCR检测 INH B MRNA的表达。结果:各组细胞活性比
较,差异无统计学意义(F=2.451,P=0.064);各组细胞 INH B MRNA的相对表达量比较,差异有统计学意义(F=
9984.370,P<0.001),酷酸铅浓度在10和10mol/L时,睾丸支持细胞细胞 INH B MRNA的表达水平低于对照
组(P<0.05)。结论:醋酸铅对大鼠睾丸支持细胞可能有毒性作用。
Effects of lead acetate on expression of inhibin B MRNA in rat Sertoli
cells
SU Xiaodong), L/ Chunyang, GAO Huiyan), ZHAO Shiguang, CHEN Xiaoyu)
7 Department of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou 2)De-
partment of Occupational Medicine, College of Public Health, Zhengzhou University, Zhengzhou 450001
3 )Center for Disease Prevention and ontrol of Quzhou City, Quzhou 324000
Key words lead acetate; Sertoli cell; inhibin B; rat
Abstract Aim: To investigate the effects of lead acetate on the cell activity and the expression level of inhibin B
(INH B) MRNA in Sertoli cells of rats. Methods The Sertoli cells were allocated into 5 groups and given lead acetate at 0
10,107, 10, and 10"mol/L, respectively. MTT method was used to observe the cell activity. The Sertoli cells were al-
located as above, and the INH B MRNA level was measured by RT-PCR. Results: The cell activity of Sertoli cells had no
significant difference among the 5 groups(F=2.451, P=0. 064).The expression level of INH B MRNA had significant
difference among the 5 groups(F=9.370, P<0.001), and the expression levels of INH B MRNA in 10 and 10
mol/L groups were higher than that of control group(P<0.05) Conclusion Lead acetate could affect the expression level
of INH B MRNA in Sertoli cells which has toxic effects on the Sertoli cells
铅是人类最早开始使用的重金属之一。随着现mRNA水平的影响,报道如下。
代工业和交通业的迅猛发展,铅的大量广泛使用,使
其已成为严重的环境污染物。铅能对机体多个系究1材料与方法
和器官造成损伤,并能影响动物和人类的生殖功
实验动物和主要试剂18~20日龄普通级雄
能2?。睾丸支持细胞在生精过程中起着非常重要性SD大鼠4~6只,由河南省实验动物中心提供。
的作用,其重要产物抑制素B( inhibin B,INHB)能DMEM/F12培养液( Gibco公司),胎牛血清(Hy-
反映睾丸支持细胞的功能状态。作者观察了不 clone公司),胶原酶I( Sigma公司),胰蛋白酶
同浓度醋酸铅对大鼠睾丸支持细胞活性及INHB( Amresco公司), HEPES培养基( Amresco公司)
甲基亚砜(DMSO, Sigma公司),其他试剂均为市售
*河南省自然科学基金资助项日0611045300
分析纯。目的基因INHB(扩増产物大小为253bp)