醋酸铅棉球法筛选口腔产硫化氢细菌的研究.pdf

国际口腔医学杂志第37卷5期2010年9月WWW.gjkqyxzz.cn
醋酸铅棉球法筛选口腔产硫化氢细菌的研究
孙崇奎,卢礼兵,朱硃,龚其美,肖晓蓉,肖丽英,周学东,李燕
(口腔疾病研究国家重点实验室,四川大学四川成都610041)
[摘要]目的对口腔常见细菌的产疏化氢能力进行筛选,期塑找到主要的口臭致病菌,分析致臭菌与龋病和
牙周病的关系。方法以醋酸铅棉球法间接定性链球茵属、放线菌属、乳杄菌属的8种可疑致龋菌以及卟啉单
胞菌属、普雷沃菌属、梭杆菌属等9种牙周可疑致病菌产硫化氢的能力。结果9种牙周病可疑致病薗均可产
生硫化氯。在致龋菌中,格氏链球菌、内氏放线菌、溶牙放线菌、酸乳杆菌产硫化氢,变异链球菌、表兄链
球菌、黏性放线茵和发酵乳杆菌不能产生硫化氢。结论产硫化氢的细菌大多数为革兰阴性厌氧菌,致臭菌与
牙周病、牙髓病的发生发展存在着相关性。约半数的致龋菌能产生硫化氯,导致口吳的发生。
关键词]硫化氢;醋酸铅;口臭:致龋菌;牙周病
中图分类号]R780.2[文献标志码]A[doi]10.3969/isn.1673-5749.2010.05.004
Preliminary study of screening oral bacteria producing hydrogen sulfide by cotton balls with lead acetate
SUN Chong-kwi LU Li-bing, ZHU Zhu, GONG Qi-mei, XIAO Xio-ron XIAO Li-ying, ZHOU ue-dong L Yan.
(State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China
[Abstract] Objective To screen common oral bacteria which produce hydrogen sulfide, and expect to find the
main source of bad breath. To analyze the relationship between halitosis and oral caries, periodontal diseases
Methods Based on the color reaction between hydrogen sulfide and lead acetate, we use indirect experimental
method by cotton-wool to detect 8 bacteria as Streptococcus, Actinomyces, Lactobacillas which are about caries
and 9 bacteria as Porphyromonas, Prevotella, Fusobacterium which are about periodontal to identify the ability to
produce hydrogen sulfide. Results Al of the 9 bacteria about periodontal disease can produce H S. In the bac
teria group about caries, only Streptococcus gordonii, Actinomyces naeslundii, Actinomyces odontolyticus, Lacto
bacillus acidophilus can produce HS while Streptococcus mutans, Streptococcus sobrinus, Actinomyces viscosus
Lactobacillus fermentata can not. Conclusion Most of the bacteria which can produce HS are gram-negative
anaerobic bacteria. The bacteria that produce halitosis have relationship with the occurrence and development of
periodontal disease, pulposis. And almost one half of the cariogenic bacteria can produce hydrogen sulfide, which
can result halitosis
Key words hydrogen sulfide; lead acetate; halitosis; cariogenic bacteria; periodontal disease
口臭在日常生活中十分常见,严重地影响人青定的是,口臭与口腔细菌关系密切2?。潜伏在
们的生活质量,且发病原因具有多元性,难以治
牙面、舌背的兼性或专性细菌发酵口腔内残余物,
愈。迄今,尚无口臭的确切定义。 Miyazaki等"?诸如唾液、血液、龈汋液和上皮细胞碎片等?,进
认为,口臭是一种由特异的细菌或特异的产硫化一步分解其中的蛋白质、肽和氨基酸,产生带臭
物的基因或酶引起的疾病;曹钟义等则认为,味的挥发性硫化物( volatile sulphide compound,
口臭更多的体现为一种与多种细菌和疾病相关联VSC)8。硫化氢是细菌分解含硫氨基酸,如半胱
的症状,与多种口腔和胃部疾病密切相关。可以氨酸和蛋氨酸后产生的,与另一产物甲基硫醇
道占VSC体积分数的90%。其中,硫化氢产生的
[收稿日期]2009-10-19;[修回日期]2010-03-23
海反应式如下;
[基金项目j口腔疾病研究国家重点实验室自主研究基金资助项目
(SKLOD PI 012)
L-半光氨酸半氨酸基(细菌编丙關酸+NH+HS
作者简介]孙崇奎(1985-);男,山东人,硕士
因此,硫化氢気既是口臭气体的主要成分,也
[通讯作者]李燕,Tel:028-85501317
是口臭检测的重要指标。本研究旨在通过简便