声表面波辅助实现纸基微流分析.pdf

第25卷第6期
传感技术学报
Vol 25 NO6
2012年6月
CHINESE JOCHNAL OF SENSORS AND ACTUATORS
Jun.2012
Paper-based Microfluidic Analysis by Help of Surface Acoustic Wave
ZHANG Yue, ZHANG Anliang", ZHA Yan, WEI Yiqing, HAN Qingjiang
(Circuit and ystems Research Laboratory, School of Inormation Science and Enginering, ingbo niversity, Ningbo Zhejiang 315211, hine
Abstract: It is usually difficult for a paper-based microfluidic device to implement pretreatment
samples. A new method for fabricating paper-based microchannels was presented, by which paper-based microfluidic
analysis with pretreatment operation was demonstrated. Pattern of microchannels was designed using Protel soft and
fabricated on a copper sheet using printing circuit technology. Paraffin was coated on the copper sheet, on which a
filter paper was mounted. The paraffin was melted and penetrated into the filter paper to perform paper-based micro
channels by heating another side of the copper sheet using an electrie iron. A microfluid to be analyzed was
transported along 128 YX-1INBO; substrate to the paper-based microfluidic device by surface acoustic wave, which
was generaled by an interdigital transducer, and reacted with indicator in the detection zone of the paper channels
Glucose and protein were concurrently detected using the presented method. The work was valuable for a paper-
based microfluidic device to implement microfluidic analysis due to its simple fabrication process and pretreatment
operation
Key words surface acoustic wave; microchannel; pretreatment operation; paper-based microfluidic device
interdigital transducer
EEACC.7820
doi:10.3969/issn.1004-1699.2012.06.005
声表面波辅助实现纸基微流分析
张悦,章安良",査燕,尉一卿,韩庆江
(宁波大学信息学院电路与系统研究所,浙江宁波315211)
摘要:纸基微流器件往往难以实现样品前处理操作。提出了一种简单的纸基微通道制作方法及兼具有前处理操作功能的
纸基微流分析方法。采用 Protel设计微通道图案,采用印刷电路技术制作铜模板,并涂覆石蜡、覆盖滤纸,而后用电烙铁加热
铜模板另一侧,熔融石蜡渗透入滤纸形成纸棊微通道。制作的纸基器件放置于128°YX- LINBO3压电基片上,当其上的叉指换
能器在电信号作用下激发声表面波,输运待测微流体到达纸基骰通道入口,并进而与其检测区中指示剂进行反应,实现微流
分析。应用所提出的方法实现了葡萄糖和蛋白质的并行检测。该工作为纸基微流分析提供了一种新的纸基微通道的制作方
法,同时,也为其解决了样品前处理操作邓难的问题,具有一定的参考价值。
关键词:声表面波;微通道;前处理操作;纸基微流器件;叉指换能器
中图分类号:'TN722
文献标识码:A
文章编号:1004-1699(2012)06-0743-05
微流器件因具有样品用量少,操作简单,易于携为市场潜力巨大的即时诊断系统(POCT)所应
带等优点得到国内外专家广泛重视い。纸基微流用?。纸基微流器件的制作是纸基微微流分析前
器件是骰流控学一个新的、重要研究领域?,它不提,已报道了多种纸基微流器件的制作方法,如
仅具有极低的成本,且制作工艺简单7-8』。因而,它 Martinez"0等提出掩膜光刻法制作纸基微流器
项目来源:浙江省自然科学基金项日(Y1080118);宁波市自然基金项目(2011A610108);宁波市服务型重点建设项目(010
A00083094702)
收稿日期:2011-12-06修改日期:2012-05-02