绿咖啡豆中总绿原酸的纯化工艺研究.pdf

试验研国食晶添加剂
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绿咖啡豆中总绿原酸的纯化工艺研究
王立志,胡晓梅,付其胜,田洪…
(1.河北省天然色素工程技术研究中心,邯单郸057250
2.晨光生物科技集团股份有限公司,邯郸057250)
摘要:绿咖啡豆中的绿原酸共有九种同分异构体,所有同分异构体的总和称为总绿原酸,研究的主要
目的是通过优化大孔吸附树脂纯化工艺将绿咖啡豆提取物中的总绿原酸含量由25%提高至70%以上,通过实
验筛选合适的大孔吸附树脂,并优化树脂纯化的工艺参数如:上柱液浓度、上柱流速、解析溶剂度数、解析
溶剂用量、解析流速等工艺指标,建立优化的纯化工艺路线。结果表明:上柱总绿原酸浓度为8mg/mL,上
柱流速为1.0BV/h,解析用乙醇浓度为70%(v/),乙醇用量为2BV,解析流速为1.5BV/h,经纯化后的总
绿原酸经HPLC检测,总绿原酸含量可达71.23%,回收率可达87.46%
关键词?绿咖啡豆总绿原酸;大孔树脂:纯化
中图分类号:R284.2文献标识码
文章编号:1006-2513(2014)07-012.3-06
Studies on the purification technology of total chlorogenic
acid in green coffee beans
WANG Li-zlhi'", HIU Xiao-mei2, FU Qi-sheng,ュ N Hong…2.°
(1. Natural Pigment Engineering Technology Center in Hebei Pr
once
Idan
057250;
2. Chenguang Biotech Group Co, Ltd, Handan 057250)
Abstract h orogenic acid in gre n coffe beans of has a olal o nine isomers, all ol lhc isomers together are called
total chlorogenic acid. The main purpose of this study is through the optimization of purification process by macroporous
adsorption resin, total chlorogenic acid content increased from 25% to 70 above. First, suitable macroporous ad-
sorption rcsin was sere ncd, then, the optimized purification process parameters such as cone ntration, low velocily
through the column, the column fluid solvent concentration and the amount were dertermined. Results showed that the
total chlorogenic acid concentration on the column was 8 mg/ml, the velocity 1.0 BW/h, elude ethanol concentration
70%(/v), the amount 2 BV, ethanol analytic velocity of 1 5 BV/h. After purification, the total chlorogenic acid
was detected by HPI C. The lola chorogenic acid contenl was up Lo 71. 23 and the recovery rale was 87.46%
Key words green cofee cans, tolal chlorogenic acid, macroporous resin, purifieation
绿原酸广泛存在于植物中,如绿咖啡豆、杜共有9种同分异构体,所有同分异构体的总和称
仲、金银花、葵花将、牛蒡等。在植物中,目前为总绿原酸,其中以5-CQA为最多,约达到总
已发现存在多种绿原酸的同分异构体,将绿原酸绿原酸含量的60%,高锦明等人对绿原酸的分
同分异构体通称为总绿原酸。绿咖啡豆中绿原酸布及同分异构的活性做了详细的概述,绿原酸具
收稿日期:2014-06-21
通讯作者
作者简介:王立志(1986-),男,硕士,研究方向为天然植物提取分离
723
2014年第7期