活性炭吸附脱除大豆磷脂细菌内毒素的研究.pdf

2012年第37卷第2期
中国油脂
油脂安会
活性炭吸附脱除大豆磷脂细菌内毒素的研究
黄使花,吕名英,宋志华,金青哲,刘元法,王兴国
(江南大学食品学院,食品科学与技术国家童点实验,江苏无锡214122)
摘藝;用活性炭吸附去除大豆磷脂中的内毒素。以磷脂残留的内毒肃度和磷脂回收率为指标,
考察了活性炭用量、吸附温度、吸附时间对内毒脱除效果的彩响。在单因素试验的基础上,通过
正交试验,得到最佳エ艺参数:活性炭用量0.6%,吸附时间40muin,吸附温度50℃。在最佳条件
下,测得磷脂中细茵内毒素的浓度为1102×10-3EU/mL,回收率为89.65%。
关键词:大?礫脂;细菌内毒煮;活性炭;吸附;脱除
中图分类号:T645-9;TS201.6文献标志码:A文章编号:1003-7969(2012)02-0055-03
Removal of bacterial endotoxin from lecithin by activated carbon
HUANG Jianhua, LU Mingrui, SONG Zhihua, JIN Qingzhe
LIU Yuanfa, WANG Xingguo
State Key Laboratory of Food Science and Technology, School of Food Science
and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China)
Abstract The activaed carbon was used to remove he bacterial endotoxin from lecithin. The effects of
dosage of activated carbon, adsorption temperature, adsorption time on the residual endotoxin concentra
tion and recovery rate of lecithin were investigated. Based on the results of single factor tests, the orthog
onall experiment was i ized to obtain the optimal conditions as follows dosage of activated carbon 0.6%
adsorption temperature 50C, adsorption time 40 min. Under the optimal conditions, the concentration of
bacterial endotoxin of lecithin was 11.02 x 10"EU/ml, and the product recovery rate was 89. 65%
Key words: lecithin bacterial endotoxin; activaed carbon; adsorption; removal
大豆磷脂是一种植物来源的礫脂,具有脂肪和胆固醇含量低、乳化稳定性好、成本低等优点,医药
行业常用精制的天然磷脂作为制备各种药物制剂的
收稿日期:2011-06-15;修回日期:2011-12-07
乳化剂和辅料1-2。然而,大豆磷脂的细菌内毒素
作者简介:黄健花(1980),女,副教授,博士,主要从事油脂问题使其应用受到限制。细菌内毒素是革兰阴性菌
化学方面的研究工作(E-mai) huangjianhua124@ina·细胞壁上的一种脂多糖( Lipopolysaccharide,LPS)和
com, cno
[5] ARTFIN N, S00-PENG K, LONG K,et al. Modeling and
erolysis for industrial monoacylglycerol production D]. Co-
optimization of lipo me RM IM - catal zed esterification of
penhagen: Technical University of Denmark, 2008: 52-55
medium -and long -chain triacyglycerols(MLCT)usin
[8] AOCS Official Method Ce 1 62, Determination of fatty
response surface methodology J ] Food Bioprocess Techn
acids in edible oils and fats by capillary GLC[S
o,2010():325-329
[9] AOCS Official Method Te 28-64, Acid value[ S]
[6] ROBLES MEDINA A, ESTEBAN CERDAN L, GIMENEAZ [10]XU X, FOMUSO L B, AKOH C C. Synthesis of structured
GIMENEZ A et al. Lipase -catalyzed esterification of
triglycendes by lipase-catalyzed acidolysis in a packed
lycerol and polyunsaturated fatty acids fror fish and mi
bed bioreactor [J ] J Agric Food Chem, 2002, 48(1)
croalgae oils[ J] Journal of Biotechnology, 1999, 70: 379
[11]郭诤.非水相系统中脂肪憔化痛化反应的研究
[7] DAMSTRUP M L. Process develapment of enzymatic glyc-
[D]天津:天津大学,2003:1~145
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