亚硝酸盐对聚磷菌厌氧代谢的影响.pdf

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第34卷第2期
土木建筑与环境工程
Vol 34 No. 2
012年4月
Journal of Civil, Architcctural & Environmental Engineering
pr.2012
亚硝酸盐对聚僯菌厌氧代谢的影响
曾被,杨莹莹,王向东,李磊,李博晓,彭永臻
(北京エ业大学环境与能源工程学院,北京100124)
摘要:以2种强化生物除磷(EBPR)系统中的活性污泥为研究对象,考察亚硝酸盐对聚礫菌厌氧
代谢的影响,结果表明:不同EBPR系统中的聚磷菌对于亚硝酸盐的耐受能力不同。人エ配水富集
聚?菌的活性污泥,当亚硝态氮浓度超过10mg/IL时,聚磷茵吸收VFA受到抑制,PHA的合成减
少,磷酸盐的释放増加;处理生活污水的SBR短程脱氩除磷活性污泥,亚硝酸盐的浓度高达
30mg/L时,未对聚磷菌的厌氧代谢造成抑制,但引起异养反硝化菌与聚砗菌竞争VFA,导致PHIA
合成量和释磷量的减少。富集聚磷菌的活性污泥投加亚硝酸盐后P/VFA增大,说明有亚硝酸盐存
在时更多的能量用于VFA的吸收。对2种活性污泥中聚磷菌的荧光原位杂交(FISH)定量分析表
明:富集聚磷莤系统中聚菌含量达到55%,而短程脱氩除磷系统中为7.6%。
关键词:聚磷茵;亚硝酸盐;厌氧代谢;抑制;污水处理
中图分类号:X703.1文献标志码:A文章编号:1674-4764(2012)02-0132-06
Effect of Nitrite on Anaerobic Metabolism of Phosphate
Accumulating Organisms
ZENG Wei, YANG Ying-ying, WANG Xiang-dong, LI Lei, L Bo-xiqo, PENG Yong-zhen
School of Environmental and Energy Engincering, Beijing University of Technology, Beijing 100124, P. R. China)
Abstract: Two enhanced biological phosphorus removal(EBPR) sludges were used to investigate the effect
of nitrite on anaerobic metabolism of phosphate accumulating organisms(PAOS). It is found that tolerance
of PAOS to nitrite in two sludges were different. In the sludge with enriched PAOS cultures treating
synthetic wastewater, when nitrite is above 10 mg/L, it inhibits Vfa-uptake of PAOS, leading to decrease
of PHA synthesis and increase of phosphate release. In the sludge with nitrogen and phosphorus removal
by shortcut nitrification treating real domestic wastewater, anaerobic metabolism of PAOS is not inhibited
even when nitrite is 30 mg/L; however, the presence of nitrite in anaerobic period causes denitrifying
bacteria compete for limited carbon sources with PAOS, resulting in decrease of PHA synthesis and
hosphate release. For the enriched PAOS cultures, the ratio of P/VFA obviously increases when nitrite is
added in the reactor, indicating increased energy requirement for PAOS to take up VFA. The quantification
results for PAOS in two sludges using fluorescence in-situ hybridization(FISH) show that the PAOS leve
is 55%o in the enriched PAOS cultures, and 7.6% in the sludge with nitrogen and phosphorus removal by
shortcut nitrification
Key words phosphate accumulating organisms (PAOS); nitrite; anaerobic metabolism; nhibition; wastewater
disposal
收稿日期:20]1-09-1
基金项目:国家自然科学基金资助项目(50878005);霍英东教育基金会高等院校青年教师基金资助项目(121076);北京
市自然科学基金资助项目(8102005)
作者简介;曾薇(1974-),女,教授,主要从事污水生物处理研究,(E-mail)zenger_1@263.net。
彭永臻(通迅作者),男,教授,博士生导师,(E-mail)pyz(@bjut.edu.cn