QuEChERS-UPLC-MS∕MS检测畜禽组织中金刚烷胺残留.pdf

食品研究与开发
检测分析
2013年12月
Food Research And Development
第34卷第23期
37
DOI:10.3969/isn.1005-6521.2013.23.01
QUECHERS-UPLC-MS/MS
检测畜禽组织中金刚烷胺残留
李彦,关丽丽,牟妍1,孙磊丽2,梁君妮1,沙美兰,邹文晓,孙爱丽,曹鹏
(1.合出入境检验检疫局,山东?台264000;2.青岛科技大学,山东青岛264200)
摘要:建立一种Q) UECHERS-超高效液相色谱一串联质谱( QUF CHERS- UPL, C- MS/MS)检測畜禽组织中金刚烷胺残留
的方法。样品用1%乙酸乙腈溶液超声提取,提取液加入(C18和PSA填料进行基质分散固相净化,浓缩后经 Proshell
20FC-C18柱分离,用电喷雾离子源正离子多反应监测(MRM)模式进行检测。在0.2g.~1000pg.线性范围内具有
较好的线性关系,相关系数大于097。该方法检出限(1IOD)为0.6ugkg,定量限(1OQ)为2.0pgkg,回收率为70.5%
100.1%,相对标准偏差(RSD)小于10%。该方法简便快速、准确度好,适用于高禽组织中金刚烷胺药物残留的检测
关键词: Ouick,Fasy, Cheap, Rugged,Safe( QUECHERS);超高效液相色谱串联质谱;金刚烷胺;畜禽组织
Method for the Determination of Amantadine Residues in Livestock an
Poultry Tissues by QUECHERS-UPLC-MS/MS
II Yan, GUAN Li-li, MOU Yan, SUN Lei-i", LIANG Jun-ni
SHA Mei-lan .OU Wen-xiao SUN Ai-ii. CAO Peng
(1.Yantai Entry-exit Inspection and Quarantine Bereau, Yantai 264000, Shandong, China
2. Qingdao University of Seience and Technology, Qingdao 264200, Shandong, China)
Abstract: A method for the determination of the residues of amantadine in livestock and poultry tissues by
QUE CHERS extraction and ultra high performance liquid chromatograph-landem mass spectrometric method
(UPI C-MS/MS)was established. The sample was extracted with acetonitrile (containing I o acetie acid )and
followed by ultrasound. Cleanup of the extracts was processed by C18 and PSA, a modified dispersive solid
phase extraction (QUECHERS). The analysts were then separated by Poroshell 120 EC-C18 column, and finall
detected by tandem mass spectrometry in positive EST mode. The linearity of amantadine in the range from
0.2 wg/kg Lo 100.0 ug/kg had correlation coefficient greater than 0.997. The limits of delection (LOD )of the
method were 0.6 wg/kg and the limits of quantification(.0Q)were 2.0 w/, The average recoveries of amantadine
were in the range from 70.5 o to 100.1. with relative standard deviations were less than 10 %. The established
method is sensitive and of good recoveries. It applies to the determination of the residues of amantadine in
poultry tissues and meat
Key words: Quick, Easy, Cheap, Rugged, Safe(Qul Che RS); ultra high performance liquid chromatography
tandem mass spectrometry; amantadine; livestock and poultry tissues
金刚烷胺是最早用于抑制流感病毒的抗病毒药,因其在体内降解代谢的量极微,主要以原形随
体液排出,若长期食用残留量高或被此类药物污染的
基金项日:山东检验疫局科技计划项日(Nws20173期台市科食品,会在人体内积累易致蓄积中毒,对中枢神经系
技发展计划项目(No.2011052)
作者简介:李彦(1989-),男(汉),助理工程师,学士,研究方向:食
统造成不良影响。金刚烷胺足国家明令禁止向食品
品安全
中添加的禁用物,口本也于2013年2月15口发布通
通信作者
知,在进口食品监控计划中加入对中国产鸡肉中金削