邻二甲苯高效降解菌的分离及其降解特性.pdf

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2016年第35卷第2期
CHEMICAL INDUSTRY AND ENGINEERING PROGRESS
565
研究开发
邻二甲苯高效降解菌的分离及其降解特性
陈佩,颜家保2,武文丽,胡茜茜
(武汉科技大学化学工程与技术学院,湖北武汉430081;2武汉科技大学教务处,湖北武汉430081)
摘要:以邻二甲苯作为唯一碳源:从长期受焦化废水污染的土壤中分离得到一株能降解邻二甲苯的高效菌株LJ5,
经生理生化和16 S RDNA鉴定,该菌株属于假单胞菌属( Pseudomonas sp.)。菌株LJ5适宜的降解条件为:培养
温度35℃,pH=7,0~8.0,摇床转速150r/min。菌株LJ5在邻二甲苯浓度高达2500mgL时,对其的去除率在30h
内可达到73%,它对邻二甲苯的降解过程符合 Monod动力学方程,当邻二甲苯浓度远远大于64.32mgL时,ILJ5
降解底物速率最高,达到43.29mg(Lh)。菌株工J5能在实际焦化废水中很好的生长,茵液投加48h后可使COD
降解率达到34.74%
关键词:邻二甲苯;分离;降解;动力学
中图分类号:X784
文献标志码:A
文章编号:1000-6613(2016)02-0565-05
DOI:10.16085/ J. Issn.1000-6613,2016.02.035
Separation and biodegradation characteristics of a o-xylene degrading
strain
CHEN Peil, YAN Jiabao2, WU Wenli, HU Xixi
(Wuhan University of Science and Technology, Wuhan 430081, Hubei, China; Officer of Academic Affairs of Wuhan
University of Science and Technolog Wuhan 430081, Hubei, China)
Abstract: With o-xylene as the sole carbon source, a strain (LJ5 )was separated from the soil which
was contaminated in the long-term by coking waste water. This strain was identified as Pseudomonas
op. based on its morphology, and 16S IDNA gene sequence. The optimum initial temperature, PH, and
shaking speed for B1 utilizing pyridine are 35, PH=7 080 and 150 I/min, respectively. The
o-xylene degradation rate was 73% when LJ15 was cultured under o-xylene concentration of
2500m/L, and the degradation kinetics of o-xylene could be described by the Monod model. When
o-xylene concentration was >6432mg/, the degradation rate of strain LJ5 reached an maximum of
43. 29mg/(L*h). Strain LJ5 could grow well in the coking waste water which allowed the degradation
rate of COD to be 34.74%
Key words: o-xylene; separation; degradation; kinetics
焦化废水含有大量有毒有机物,如酚类、苯系降解萃系物的微生物有假单胞菌属( Pseudomonas
物及吡啶等杂环化合物り。邻二甲苯是一种典型的sp.)、红球菌属( Rhodococcus sp.)和芽孢杆菌属
苯系物污染物,由于其毒性大和难生物降解的性质
使得传统生物法对其处理效果不佳。任源等?研
收稿日期:2015-06-30;修改稿日期:2015-07-13
基金项目:湖北省自然科学基金重点项目(2011CDAO54)、武汉科技
究发现焦化废水经生物处理后的出水中仍含有苯类大学研究生创新创业基金重点项目(cxo6)及武汉科技大学国家
物质,因此,向生物处理系统中投加从自然界中筛级大学生创新创业训练计划项目(2013104880
选出的高效菌叫,从而提高对目标污染物的去除率
第一作者:陈佩(1990-),女,硕土研究生。E-mai504532896@qcom
联系入:颜家保,教授,博士生导师,主要研究方向为环境友好化工
受到了国内外广大环保者的关注。目前,已报道的新技术。E-mai97278740m