联苯菊酯降解菌的筛选、鉴定及降解特性研究.pdf

农业环境科学学报2012.31(6:1147-1152
Joumal of Agro-environment Science
联苯菊酯降解菌的筛选、鉴定及降解特性研究
刘婷婷,董昆明,缪莉,周晓见,新翠丽1,姜薇,封克2
(1.扬州大学环境科学与工程学院,江苏扬州225127:2.江苏扬州农业环境安全服务中心,江苏扬州225127)
摘要:联苯菊酯是一种广谱高效杀虫剂,大规模的应用使其广泛残留在环境中,因此筛选联苯菊的高效降解菌具有重要意义。
从扬州农药厂附近的地表士壤取样,利用高集驯化培养分离得到一株编号为S8的降解细菌,经表形特征、生理生化特性和16S
rDNA序列分析其为带酸钙不动杆菌( Acinetobacter calcoaceticus),该菌株在pH7.0和30℃的条件下,对100mg'L联苯菊酣的3d
降解率达564%,半衰期为60.7h。其最适生长条件为:pH60-8,0,温度30-35℃,接种量5%。研究结果可为今后治理联苯菊酯残留
污染提供理论参考。
关键词:联苯菊酯;降解菌;降解特性
中图分类号:X172文献标志码:A文章编号:1672-2043(201206-1147-06
Isolation, Identification and Biode radation Characteristics of a Bacterial Strain able to Degrade Bifenthrin
LIU Ting-ting DONG Kun-ming MIAO Li ZHOU Xiao-jian, JIN Cui-, JIANG Wei, FENG Kela
(1. Institute of Environmental Science and Engineering. Yang hou University, Yan zhou 225127. China: .Yangzhou Technolo ical Service
Center for Agro-environment Safety of Jiangsu Province, Yangzhou 225127, China)
Abstract Bifenthrin( BF )is a broad-spectrum and highly efficient synthetic insecticide However the difficulty of its natural degradation
makes it widely remain in the environment, which causes many critical environmental problems. Microbial biodegradation of pesticide
residue especially baea de daion s e main way in epair of such o f enviroment polution, Although here have been som
reporsonthepyrethroidinseticidedegadationbaeteria,thedegradationefectandstabiliryofthoeebacteriastilnedtobeexplored
Therefore the screen for efficient BF degrading bacteria has great research significance. In this study, a BF degrading bacterium designated
as $8 was solae pui e and m ee l r o e Yn ou esti e Plant. The acteria train S was identified as
Acinetobacter calcoaceticus acing o is morphological h il al and chemcal characterization as wel as the sequence analysis
of its I6S DNA. The BF degradation eached equilibrium at 7 h and the degradation rate was 56. 4%o initial concentration of 100m L.)
with half lives of 2. 53d under e eon ons of H7. and 30 .T pma ntns or B de ion were al PH6.0-8.0, 30-35C
and with 5% inoculation. The results indiate that the Be ng stain$ had eat appli ation potential for the future bioremediation of
environmental contamination by BF.
Keywords: bifenthrin; degrading bacteria; biodegradation characteristics
联苯菊酯是20世纪80年代初开发的一种新型家庭有害昆虫的防治。该化合物对光、热稳定,能附着
杀虫剂,具有强大的胃毒和触杀作用,广泛应用于对作物表面不易被雨水冲刷,故残效期长,进人环境不易
棉花、蔬菜、果树、茶树等害虫的防治及白蚁叫螨虫等分解叫。据相关文献报道,联萃菊酯在土壤中的消解过
程与施药浓度、土壤类型以及土壤含水率有关,在含
收稿日期:201-11-23
水率20%的沙质和有机质土壤中,联苯菊酯的半衰期
基金项目:国家自然科学基金项目(41076097,41006097,41106113);
江苏省自然科学基合(BK2010322);江苏省属高校自然科
分别为411d和522d,而在.水浸没时两类土壤中的半
学研充项目(10KJB17003):扬州大学科技创新培育基金衰期大致相同为267門。长期使用势必会在环境中造
作者筒介:刘婷婷197ー),女,江苏扬州人,预1研究生,研究方向为大量残留,而联苯菊酯对哺乳动物具有神经毒性,
2011CX032
环境科学。E-mait: tingtingliult17 a gmail. com。
对蜜蜂具有高强毒性,尤其是对鱼类以及水生生物有
通讯作者:昆明E-mail:kedong@yzr.echu.cn
很强的毒性并能干扰内分泌,有抗雄激素作用?。美
万方数据