果汁中菌落总数的不确定度评定.pdf

第7卷第6期
食品安全质量检测学报
Vol 7 No. 6
2016年6月
Journal of Food Safety and Quality
果汁中菌落总数的不确定度评定
汪艳玲,赵怀荣
(准安市疾病预防控制中心,准安223001)
摘要:目的建立评定菌落总数不确定度的方法,以减少实验误差并提高检测结果的精确度。方法按照
GB4789.2-2010《食品安全国家标准菌落总数测定》檢测20份果汁样品中菌落总数,依据JF1059.1-2012《测
量不确定度评定与表示》分析菌落总数的不确定度来源;采用合并样本标准差计算,评定檢测结果的不确定
度。结果在包含概率为95%时,果汁中菌落总数的扩展不确定度为0.10(以对数计),此结果适用于同类样品
的检测。结论用合并样本标准差评定多个同类样本菌落总数的不确定度较为方便,随着检验数量的不断增加,
数据可随时加入到合并样本中,从而对菌落总数的不确定度进行合理判定,从而対食品进行正确的卫生学评价。
关键词:食品安全;菌落总数;不确定度
Uncertainty evaluation of aerobic plate count in fruit juice
WANG Yan-ling, ZHAO Huai-rong
(Huaian City Center for Disease Control and Prevention, Huaian 223001, China)
ABSTRACT: Objective To establish a method for evaluating measurement uncertainty of the colony determination
so as to decrease the experimenta error and increase the accuracy of measurement Methods According to (B
4789.2-2010 National food safety standard Food microbiologica examinato deric plate count, 20 fruit juice
samples were measured. According to JF 1059.1-2012 Evaluation and expression of uncertainty in measurement. the
source of the uncertainty in the detection was analyzed. The method of merging sample standard deviation was
applied to determine the uncertainty evaluation. Results As the coverage probability was 95, the expanded
uncertainty of fruit juice was 0.10, which could be adopted to the same kind of samples. Conclusion The method of
merging sample standard deviation is convenient for uncertainty evaluation of aerobic plate count of multiple similar
samples, complicating sample standard deviation must be calculated again when more results were added, and the
extent of uncertainty has to be renewed. The method can be used for uncertainty evaluation of aerobic plate count
dctection, so as to assess the quality of microbiological detection correctly
KEY WORDS: food safety; aerobic plate count; uncertaint
监管,对维护人民群众身体健康和生命安全具有重要意义
食品中徽生物的检测是评价食品安全的重要卫生指标,菌
新《食品安全法》的正式实施对食品安全风险监测提
落总数是最常见的检验项口,主要用来判断食品被细菌污
出了更高的要求。开展食品中污染物的污染状况监测,分的程度,可川チ该食品进行生学评价。出于所有的
析和评价食品污染物数据,开展风险监测顸營和实施政府
测量都存在误差,存在檢测结柴是否能客观评判的风险,并
通讯作者:汪艳玲,主管技师,主要从事微生物檢验工作。E-mai:wangyll982@163.coma
Corresponding author: WANG Yan-ling, Supervising chian Huaian ity enter for Disease Control and Peven n, Huaian 223001, China
E-mail:wangyll982(@163.com
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