琥珀酸放线杆菌发酵产琥珀酸的优化.pdf

食品与皮科技
ermentation Technolog
第48卷(第3期)VoL.48,Nn.3
琥珀酸放线杄菌发酵产琥珀酸的优化
周彬,华沸文,周胜德,赵锦芳,王金华
(湖北工业大学生物工程学院,湖北武汉430068)
搞要:本文通过响应曲衝法(RSM)达到优化珀酸放线杆菡培养基分的目的。进行单因煮实验确定显著因煮后
采用响应面法倪化魏珀酸放线杆菌 CGMCC1593的发酵培养条件。利用 Designー Expert软件对倪化结果进行回归分
析。单因素实验确定葡糖浓度为40gL,酵母粉浓度为20gL,温度为373℃,pH为75,相应面法优化得到葡萄糖浓
度,酵母膏浓度,温度,pH,分别为39.1g/L,20.5gL,37.3℃,7.6,在此条件下號酸的产量达到37.2gL,比优化前捉高
了10.1gL,与模型预测的37,4gL基本吻合,葡糖和pH交互作用不明显(P>0.05),葡萄糖、酵母粉和pH的交互作用
均显著(P关词:琥珀酸放线杄菌;琥酸;相应面优化
中图分类号:1Q921+.7
文标识码:A
文章编号:1674-506X(2012)03-0018-0004
Optimizing the Conditions of Succinate Fermentatation by Actinobacillus
Succinogenes
ZHOU Bin, HUA Bo-wen, ZHOU Sheng-de, ZHAO Jin-fang, WANG Jin-hua
( School of biotechnology, Hubei University of Technology, Wuhan 430068, China
Abstract Response surface methodology (RSM)was used to optimize the fermentation condition of Actinobacillus
Succinogenes. Based on confirming sigificant factors through single factor experiment, the fermentation medium of Act-
nobacillus succinogenes GMCC1593 was optimized by response metheod and the quadratic regression analysis was
conducted on the optimized esults by using Design-expert software It was confmed through single factor experiment
that the carbon source glucose was 40/L, nitrogen source yeast extract was 20/L, temperature was 37C and the PH
was 7. 5. The optimum results of 4 signi cant actos of gluse, east extract, emperature and PH obtained through
optimization by response surface method were 39.1/L, 20.5 L, 373C, 7.6 resp. Under this condition, the output of
succinate was up to 37.2, increasing by 10.1 L than that before optimization and basically according with the model
predication value of 37. 4 The interaction between glucose and pht was not significant (P>0.05 )and that between
glucose, yeast extract and PH were significant(P<0.05 The output of succinate after the fermentation medium of A
succinogenes was optimized by response surface method was enhanced by 37.5% than that before optimization
Key words Actinobacillus succinogenes; succinate; response surface metho
doi:10.3969/issn.1674-506X.2012.03-005
琥珀酸即丁二酸,是重要的有机化合物,被广泛键化合物,是合成众多重要工业化学制品的中间物
的应用于医药、农药、染料、香料、油漆、食品等行业质。琥珀酸作为优秀的C4平台化合物,其传统的
材料工业。因其是合成30多种重要商业产品的关生产方法是石化发,由丁烷通过顺式丁烯二酐生产
收稿日期:2012-03-07
基金资助:湖北省教育厅湖北工业大学高层次人オ启动基金,2011
作者简介:周彬(1983-),硬土研究生,研究方向为徵生物发酵及应用
通讯作者:王金华(1964-),教授,硕士生导师。研究方向:生物技术及生物质能源。 wangjin hua@ maill. abut.edu.cr