高效液相色谱法检测饮料中艾德万甜含量.pdf

第7卷第12期
食品安全质量检测学报
Vol. 7 No 12
2016年12月
ournal of Food Safcty and Qualily
I)cc..2016
高效液相色谱法检测饮料中艾德万甜含量
陈、健',林真2,钱疆,张玉燕!,杨方
福建出入境检验检疫局裣验检疫技术研究重点实验室,福州35000
2.福建生物工程职业技术学院,福州350002
摘要:目的建立饮料食品中艾德万甜的高效液相色谱检測法。方法选择 Agilent'/ orban X)BC1s色谱柱、
对实验的检测器、流动相和柱温进行选择。并对方法的回收率和精密度进行分析。评价方法的可行性。结果选
择荧光检測器进行检测,检测波长为:激发波长230nm,发射波长310nm;流动相为甲醇水溶液(50:50,1:レ
柱温为25℃,流速为0.8 ml in。在所确定的实验条件下,艾德万甜标准溶液在0.05-5.0mgl.范围内呈良好
的线性关系,线性系数大于0.99平均回收率为85.090~-102.0%,相对标准偏差均小于5.09%。结论该方法简
单、精密度和准确度良好,可以满足实际检测的需求。
关键词:高效液相色谱法;艾德万甜;饮料:荧光检测器
Determination of advantame in beverage by high performance liquid
chromatography
Chen Jian, Lin Zhen, Qian Jiang, Zhang Yu-yan, Yang Fang
(1. Key /aboratory o/Inspection amd aramine Yechnology Cener o/Hujian Enlry-fxil Inspection and uarantine
Bureatl, Fiehou 350003, China: 2. Fujian Vocational College of Bioengineering, Fuzhou 350002, China)
ABSTRACT: Objective To establish a method for the determination of advantame in beverage by high
performance liquid chromalography (IIPL. C). Methods The detector, mobile phase and column temperature werc
selected for the experiment using an Agilent /orbax XIB Cus column. The recovery rate and precision of the method
were analyzed to evaluate the feasibility of the method. Results The fluorescence detector(FLD)was selected for
the detection with the excitation wavelength o! 230 nm and the cmission wavelength of 3 10 nm. The mobile phase o!
the experiment was methanol-water solution(50: 50. 1: 1), the column tcmperature was 25 C, and the Tlow rate was
0. 8 m/min. In the determined experimental conditions, advantame standard solution had a good linear relationship in
the range of 0.05-5.0 m9/L with the linear coefficient greater than 0.099. The average recoveries were
85.0%0--102.0%, and the relative standard deviations(RSDS)were all less than 5.0%. Conclusion Ihe cstablished
method is simple, precise and accurate, which can be used for the determination of advantame in realit,
KEY WORDS: high performance liquid chromatography, advantame; beverage; fluorescence detector
基金项目:认监委标准资助项目(SNT3854)、国家质检总局科技项目(2014IK097)
Fund: Supported by Standard Funding Program of cification and Accreditation Adminstration of the Icoplcs Rcpublic of hina(SN/ 3854)and
Scicncc and Technology Roject of Gencral ministration of Quality Superision, Inspection and Quarantine of the Pcoplcs Rcpublic of China
(2014K097
通讯作者:杨方,研究员、主要研究方向为食品理化分析检測。T-mail:ylljeiq(agmail.com
orresponding author: YANG Fan, rofssor Tcchnical nter of Fu an Entry-exit Inspction &Quarantin Burcau, No 312, Hudong Road,
Fuzhou 350003, China. E-mail: yftjciq(Gmail. com
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