应用透光度比较两种根管消毒药对粪肠球菌的抑菌效果.pdf

Chinese General Practic
Ju201y?J7
短篇论著
应用透光度比较两种根管消毒药对粪肠球茵的抑菌效果
于静
摘要】目的比较两种根管消毒药对粪肠球菌的抑菌效果。方法选取40颗新鲜拔除的人单根管牙,截取
40个6m高的柱状牙根,将牙根随机分为4组,每组10颗。清理根管后灭茵制备感染粪肠球茵根管模型,阴性对照
组根管不感染粪肠球茵,放置0.9%氯化钠溶液;其余3组根管均感染粪肠球菌,分别放置甲醛甲酚液(PC组)、丙
二醇-氢氧化钙糊剂(丙二醇一氢氧化钙糊剂组)及0.9%氯化钠溶液(阳性对照组)。3d、7d后分别培养并测量细
茵的透光度,比较4组细菌的透光度有无差异。结果4组培养物培养3d细菌透光度比较,差异有统计学意义(P
0.05);其中丙二醇一氯氧化钙糊剂组与FC组比较,差异无统计学意义(P>0.05)。7d时4组细菌透光度比较,差
异有统计学意义(P<0.05);其中丙二醇一氯氧化钙糊剂组与FC组比较,差异无统计学意义(P>0.05),该两组与
阴性对照组比较,差异无统计学意义(P>0.05)。结论丙二醇一氩氧化钙糊荆与甲醛甲酚液对粪肠球茵均有较好的
护菌效果。
关键词】根管;抑菌效果;消毒剂;肠球菌,粪
中图分类号】R378.1【文献标识码】B【文章编号】1007-9572(2010)7B-2266-03
Using Transmittancy to Compare Antibacterial Activities of 2 Kinds of Root-canal Disinfective Drugs in Enterococcus
Faecalis U Jing, Department of Stomatology, Second Hospital of Shijiazhuang City, Shijiazhuang 050051, China
Abstract] Objective To compare the antibacterial activities of 2 kinds of root-canal aisinfective drugs in Enterococ
cus facealis. Methods Forty 6-millimeter-high cylindrical roots from newly-extracted human single canal teeth were divided
randomly into 4 groups, 10 in each. The root canals were sterilized to establish Enterococcus faecalis infected models after clean-
ing. Negative control group not infected with Enterococcus faecalis, and put in.9% sodium chloride solution, the other 3 root
canals infected and put, respectively, in formaldehyde cresol solution(FC group), propylene glycol-calcium hydroxide paste
group PG-CHP)and 0.9% sodium chloride solution(positive control group). On days 3, 7, bacterial transmittancy were
determined. Results There was significant difference in bacterial transmittance between 4 groups after 3 d of culture(P
0.05), but group PG-CHP were not different from group FC(P>0. 05). There was significant difference in bacterial transmit-
tance between 4
after 7 d of culture(P<0.05), but group PG-CHP was not different from group FC(P>0.05), and
the 2 groups not different from negative control group(P> 0.05 Conclusion Propylene glycol-calcium hydroxide paste and
FC have good antibacterial effects.
Key words) Root canal Inhibitory effect; Disinfectants; Enterococcus faecalis
口腔内科常见的牙髓疾病和根尖周病粪肠球蘭的抗菌活性,并比较两种消毒药自动立式蒸汽灭菌器( Model IS-B75Lー
的最主要病因是细菌感染,根管治疗术是对粪肠球菌的抑菌效果。
Ⅱ,江阴滨江医疗设备有限公司);电热
治疗此类疾病最根本、有效的方法。但该1材料与方法
恒温培养箱(山东省潍坊医疗器械厂);
方法仍有20%~30%的失败率。而粪肠1.]材料及主要设备
数字显示电子比浊仪(100%T,上海复
球菌( Enterococcus faecalis,F. faecalis)1.1.1实验材料丙二醇-氢氧化钙糊星佰珞生物技术有限公司);一次性使用
是根管内再感染最常见的细菌。根管消毒剂(解放军第105医院口腔医疗中心配细菌培养皿(直径为90mm,江苏康健医
是根管治疗术的重要步骤之一,其消毒效方);FC液(武汉大学口腔医学院口腔疗用品有限公司);生物显徴鏡(OLYM
果主要取决于根管消毒剂。本研究通过在药物材料厂,批号:09017);无菌PUS-212,日本奥林巴斯仪器公司);
离体人牙根管内接种类肠球菌,建立口腔0.9%氯化钠溶液(石家庄四药有限公15°~40'且型根管锉(MANI,日本)
内感染根管模型,滉察感染根管内甲醛甲司,批号:091019107);普通血琼脂培养1.2交验菌种粪肠球菌临床菌株1株
酚(FC)液及丙二醇一氢氧化钙糊剂对基(90m,郑州安图绿科生物工程有限石家庄市第二医院细菌室提供。
公司)。
1.3标本及分组
作者单位:05001河北省石家庄市第二医1.1.2实验设备电子分析天平1.3.1标本选择2009年6-8月我院
院∏腔科
(MA240D型,上海第二天平仪器厂);全日腔科因正畸治疗拔除的40颗新鲜人单