丁草胺和苄嘧磺隆对钝顶螺旋藻的毒性效应研究.pdf

农业环境科学学报201130(60:1070-1075
Journal of Agro-environment Science
丁草腔和苄嘧磺隆对钝顶螺旋藻的毒性效应硏究
贺鸿志2-,余景,骆世明,李拥军4,邱晓辉13,罗琼珠,黎华寿1,3
(1.华南农业大学农业部生态农业重点开放实验室,广州510642;2.广东省渔业生态环境重点实验室,广州510300;3,广东省高
等学校农业生态与农村环境重点实验室,广州510642;4.中山市农产品质量监督检验所,广东中山528403)
橢要:采用实验室摇瓶实验法研究了除草剂丁草胺和苄嘧磺隆对钝顶螺旋藻的毒性效应。结果表明,丁草胺浓度与藻生长(以
ODs计)存在明显的剂量一效应关系,64mg?L-丁草胺严重抑制藻生长,比生长速率为0.11-,仅为对照的24%。在7d实验时间
内EC值随染毒时间延长呈指数下降,96h的EC值为45.4mg“L,到第7d降为152mgL-。与丁草胺相似,在实验浓度范围内
随浓度增加,苄嘧磺隆对饨顶螺旋藻的生长抑制作用逐渐增强,最高浓度4.8mg?L处理的比生长速率为0.458d-,约为对照的
82%。在8d实验时间内ECn值随染毒时间延长呈指数增长,9%h的ECo值为51.3mgLH,到第8d达到215mg-L。两种除草剂在
较高浓度条件下均使钝顶螺旋藻藻丝变短,部分藻丝由螺旋型变为直线型,随浓度增加直线型藻丝逐渐增加,到最高浓度几乎全部
变为直线型,且藻细胞颜色变浅。依据9%6h-FC。值判断丁草胺对钝顶螺旋藻的毒性大于嘧磺隆,二者毒性强度均为中等,但随着
染毒时间廷长丁草胺的毒性作用增强而苄嘧磺隆的毒性逐渐降低。同时,高浓度的两种除草剂使部分藻丝断裂变短,且诱导藻丝形
态由螺旋型向直线型转变
关键词:丁草胺;苄嘧磺隆;饨顶蠔旋藻;毒性
中图分类号:X592文献标志码:A文章编号:1672-2043201106-1070-06
Toxic Effects of Butachlor and Bensulfuron Methyl on Cyanobacterium Spirulina platensis
HE Hong-zhi, YU Jing, LUO Shi-ming, LI Yong-jun,QIU Xia- hui, LUO Qiong-zhu-, LI Hua-shoul
1 Key Laboratory of Ecological Agricul ure of Minis of culture South China Agricultural University, Guangzhou 510642, China; 2.
Key laborator of Fishery Ecology and Environmen Guangong Province, Guanghou 510300, China 3 Key Laboratory of Agroecology and
Rural Environment of Guangdong Regular Higher Education Institutions, Guangzhou 510642 China; 4. Zhongshan Quality Supervision
Inspection Institute of Agricultural Products, Zhongshan 528403, China
Abstract: The oxic effects of utachlor and bensul uron methyl on Platensis were studied using shaking flash method. It showed that sig
nificant do age ef c of u achor on agal ow h( ed in D )ex ed The wh of S platensis exposed under 64 mg L butachlor
was severely ibi ed eifc w h a was 1 was o 4 of e cont. Dung the 7d exprimental perod, Decreased
exponentially. The EC in 96 h was 45. 4 mg and it drop ed to 15.2 mg, in day Like utachlor, dosage effect of bensulfuron methyl
on growth inhabit ation on S pl sis was also si ifican pecific owth rae under448 mg ament was 0. 458 -. It was82%of h
con. During the &d expe men a period, EC value increased exponentially. E in 96 h was 51.3 mg L, and EC in day8 was 215mg
When reated with these two herbicides, alga filaments of S platensis became shorter, and some of the spiraled filaments changed into a
linear form. Percentage of linear algal flaments increased while herbicides concentration increased. Nearly all algal flaments changed into
linear om with much lig olo n e agal ulte with the hi est concentation of herbicides. Although toxicity of the two herbicides to
S. platensis was moderate, butachlor exhibited higher toxicity than bensulfuron methyl to the alga according to 96 H-EC With longer expo-
sure time, toxicity of butachlor increased while that of bensulfuron methyl decreased gradually. Lots of spiraled filaments of S.platensis
switched to linear ones under high concen ration reatments, and some filaments even disconnected and became much shorter.
Keywords: butachlor; bensulfuron methyl; Spirulina platensis toxicity
收稿日期:2010-11-29
基金项目:国家自然科学基金(310004,30870413);广东省渔业生态环境重点实验室开放基金(LFE-2009-07);广东省自然科学基金
(9451064201003803);华南农业大学校长科学基金(2008K022)
作者简介:贺鸿志(1977ー),男,湖南娄底人,博土,讲师,主要从事污染生态学研究。E-mail:cauh@can.edu.cn
通讯作者:黎华寿E-mail.lihuashou@scau.edu.cn
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