木聚糖提取优化及对木聚糖酶活性的影响.pdf

食品研究与开发
2017年8月
分离提取
Food Research And Development
第38卷第16期
DOI:10.3969/.iss.1005-6521.2017.16.015
木聚糖提取优化及对木聚糖嗨活性的影响
周义,张东杰,李超楠宋雪健,于果,于金池
(黑龙江八一农垦大学食品学院,黑龙江大庆163319
摘要:以小米槺为试验原料,研究小米糠中木聚糖的提取工艺及对肠道木聚糖酶活力的影响。在单因素的基础上,
采用正交试验优化小米糠木聚糖提取工艺,研究料液比、碱提温度、碱液浓度、碱提时间4个因素对提取木聚糖提取
率的影响。并采用着色木聚糖标记法測定木聚糖酶活力,研究小米槺木聚糖对大鼠肠道粪便及消化道木聚晦活力
的影响。结果表明,小米糠木聚檐的最佳提取工艺:料液比为1:25(gm),碱处理温度为100℃,碱液浓度为10%,碱
处理时间为2h,在此条件下木聚糖提取卒为21.71%
关键词:小米糠;木聚糖;着色木聚糖标记法;木聚糖酶活性
Extraction of Xylan and Its Effect on Xylanase Activity
ZHOU Yi, ZHANG Dong-jie, Ll Chao-nan', SONG Xue-jian, YU Guo, YU Jin-chi
College of Food Seience, Heilongjiang Bayi Agric
ultra Driver
rsity, Daqing 163319, Heilongjiang, China
Abstract Small rice bran as raw malerial lesting, research in rice bran extraction process small impact on the
xylan and xylanase activity of the intestinal tract. On the basis of single factor, orthogonal experiment was use
to optimize the extraction proeess of millet bran chitosan. The effects of four factors, such as ratio of material to
liquid, alkali temperature, alkali concentration and alkali extraction time, on the extraction process were stuc
ied. And the xylanase activity was measured by the method of coloring xylan labeling to study the effects of millet
chaffidan on intestinal fecal and xylanase activity in digestive tract. The results showed that the best extraction
process was as follows: the ratio of material to liquid was 1: 25( /ml), the alkali treatment temperature was 100
C, the alkali concentration was 10 %, the alkali trealment time was 2 h, and the xylan was rate of 21.71 %o
Key words: millet bran; xylan; coloring xylan labeling method; xylanase activity
谷子是我国东北及西北地区主要杂粮作物,种植膳食纤维等。但由于小米糠的外观差、有异味和缺乏
面积达1400千亩,年产量370万吨~450万吨。小米可食性等原因,使小米糠在实际应用中主要被用作动
糠是由褪下的谷壳、种皮、糊粉层和米胚芽组成,占谷物的饲料,这样不仅造成了资源的严重浪费,而且对
子重量的5%~7%?,小米糠作为谷子加工过程中产生环境还会产生不良影响。为提高小米糠的利用价
的副产物之一,是一种产量较高的可再生资源,并含值,研究从小米糠中提取木聚糖的工艺具有一定的研
有丰富的营养成分,如蛋白质、维生素、脂肪、矿物质和究意义。日前,从农作物中提取木聚糖的方法主要有
作者简介:周义(1992-),男(满),研究生,研究方向:农产品加工及
超声波辅助法、超声处理结合蒸煮法?、碱性过氧化
心工程
氢等,但由于这些方法并不成熟,现还以碱提一蒸煮
通信作者:.张东杰(196-),男、教授,博十,研究方向:农产品加工法为主。植物组织内天然结构的木聚糖受到木质素
与安全。
屏蔽而很难被晦解。即使在牛科动物瘤背中木聚糖的
munications,2004,320(4):1103-111
var. gerardianus (Bentham)H. Hara [Jl. Carbohydrate Polymers
116 Lingrong Wen, Lianzhu Lin. Lltrasound-assited extraction and struc
2011,85:541-547
al identification of polysaccharides from isodon lophanthoides
收稿日期:2016-11-24
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