介孔TiO_2对溶菌酶吸附的动力学和热力学.pdf

物理化学学报( Wuli Huaxue Xuebao)
638
Acta Phys. -Chim. Sin. 2016, 32(3), 638-646
March
Article
doi:10.3866/PKU.WHXB201512181
www.whxb.pku.edu.cn
介孔TO2对溶菌酶吸附的动力学和热力学
洪启亮'董依慧1庄伟2饶超'刘畅
(南京工业大学,材料化学工程国家重点实验室,南京210009:2南京二业大学生物与制药学院,南京21816
摘要:773.15K下焙烧二钛酸(H2T2Os)制备了介孔结构TO2。采用比表面分析仪(BET)、扫描电镜(SEM)、
拉曼( Raman)光谱和X射线衍射(XRD)仪进行表征研究了介孔TO2对溶茵酶的吸附行为和机理。结果表明,
该吸附过程较好地满足 Langmuir吸附模型;随着溶液pH值的増高,溶菌酶在介孔TO2上.的吸附量先增大后
减小。在pH=7.2时,达到最大吸附容量72.5mgg。该介孔TO2对溶菌酶具有良好的吸附稳定性,经过5
次循环后吸附的溶菌酶残余量仍有81.6%。动力学研究表明,介孔TO2与溶菌酶间的吸附满足准二级动力学
模型,吸附传质过程由膜扩散和粒内扩散共同影响与控制。对热力学参数的计算发现,该过程△G<0,
△HP>0,△S>0,表明介孔TiO2对溶菌酶的吸附是一个自发的、吸热的熵增过程
关键词:介孔TO2;溶菌酶:吸附;动力学;热力学
中图分类号:O647
Kinetics and Thermodynamics of Lysozyme Adsorption on
Mesoporous Titanium Dioxide
HONG Qi-liang DONG YI-HI
ZHUANG Wei
RAO Chao
LIU Chan
(State Key Laboratory of Materials-oriented Chemical Engineering, Nanjing Technology University, Nanjing 210009, P R. China
School of Biological and Pharmaceutical Engineering, Nanjing Technology University, Naming 271876,P R. China,
stract: Mesoporous Tio was prepared by calcinating H Ti O at 773. K The sample was characterized
by Brunauer-emmet-teler(BE T) scanning electron microscopy(SEM), Raman spectroscopy, and X-ra
diffraction(Rdanalysis. The adsorption behavior and mechanism of mesoporous TIO for lysozyme were
inest y ste mal adoption experiments. The results show that the equilibrium experimental data were
correlated with the Langmuir isoterm equation. The adsorption capacity first increased and then decreased
with increasing ph value. The capacity showed a maximum value of.5 mgg when the ph value was 7.2
Lysozyme adsorbed on mesoporous Tio was extremely stable, and its amount on mesoporous Tio maintained
81. 6% of its initial value after five adsorption and regeneration cycles. Furthermore, kinetic analysis was
conducted using pseudo-first and pseudo-second order models. The adsorption of lysozyme on mesoporous
TO was descr e well by the pseudo-second order rate equation. The rate-determining step of the adsorption
was the combined action of film diffusion and intraparticle diffusion. The adsorption thermodynamic analysis
suggested AG"< 0, AH> 0, and As> 0, which indicated that the adsorption was a spontaneous and
endothermic process with entropy increased
Key Words: Mesoporous Tio2 Lysozyme; Adsorption; Kinetics; Thermodynamics
Received: September 14, 2015, Revised: December 14, 2015; Published on Web: December 18, 2015
Corresponding author. Email: changliu(njtech.edu.cn; Tel: +-86-13913939041
The project was supported by the National Key Basic Research Program of China(2013CB733501), National Natural Science Foundation of China
(21136004 21476106, 21506090) Natural Science Foundation of Jiangsu Province, China(BK201 30929), and Project of Priority Academic Program
Development(PAPD) of Jiangsu Higher Education Institutions, China.
国家重点基础研究发展计划项目(2013cB7335012国家自然科学基金(2!13600,21476106,21506090,江苏省自然科学恭合(BK20130929)和
江苏高校优势苧科建设工程项目资助
C Editorial office of Acta Physico-chimica Sinica