NVP对溶菌酶的吸附行为与吸附机理.pdf

物理化学学报( Wuli Huaxue Xuebao)
February
Acta Phys-chim. Sin, 2010, 26(2): 359-366
[Article]
www.whxb.pku.edu.cn
接枝微球PMAA- BIEMIANYP对溶菌酶的吸附行为与吸附机理
付红艳高保娇牛庆媛
(中北大学化学工程系,太原03005)
摘要:采用反相悬浮聚合法制备了甲基丙烯酸羟乙酯(HEMA)与N-乙烯基吡略烷酮(NVP)的交联共聚微球
HEMA/NVP,然后采用“接出”法,实施了甲基丙烯酸(MAA)在交联微球表面的接枝聚合,制得了接枝微球
PMA- HEMA/NVP.以溶菌酶(LYZ)为模型碱性蛋白,深入研究了接枝微球PMAA- HEMA/NVP对碱性蛋白的
吸附性能与吸附机理.测定了徴球PMAA- HEMA/NVP的zeta电位,考察了PMAA接枝度、介质pH值及离子强
度等因素对体系吸附性能的影响.结果表明,在较大的pH范围内,接枝微球PMAA- HEMA/N]P的zea电位为
绝对值较大的负值,即其表面携带有高密度的负电荷.在强静电相互作用的驱动下,接枝微球PMAA-HEMA
NVP对溶菌酶表现出很强的吸附能力.随介质pH值的增高,接枝微球对溶菌酶的吸附容量呈现先增大后减小
的变化趋势,在与溶菌酶等电点接近的pH值处φH=),具有最大的吸附容量(90mgg);离子强度对接枝微球
的吸附能力也有较大的影响,当pH<9时,溶菌酶吸附容量随NaCI浓度的増高而减小;当pH>9时,吸附容量随
NaCl浓度的増高而增大.
关键词:聚甲基丙烯酸;聚合物接枝微球;碱性蛋白质;溶菌酶;吸附机理
中图分类号:O647
Adsorption Behavior and Mechanism of Grafted PMAA-HEMA/N VP
Microspheres toward Lysozyme
FU Hong-yan GAO Ba0-jia0 NIU Qing-yuan
Department of Chemical Engineering, North University of China, Taiyuan 030051, P R China)
Abstract: The crosslinking copolymerization of 2-hydroxyet l methacrylate(HEMA )and N-vinylpyrrolidone
(NVP)was performed using the inverse suspension polymerization method resulting in crosslinked HEMA/NVP
maros. sequent the raft polymerization of methacrylic acid (MA was conducted using the raftin
from "method and the grafted PMAA-HEMA/NVP microspheres were obtained. The adsorption property and
adsorption mechanism of the grafted microspheres were investigated in depth using lysozyme(LYZ)as a basic protein
model The zeta potential of the grafted microspheres was determined. Several actors that affected the adsorption
property of the system such as the PH of the medium, the grafting degree of the microspheres and the ionic streng
were examined. Eperimental results indicate that in a wide ph range, the zeta potential of the grafted PMAA-HEMA/
NVP microspheres has a large negative value. Therefore, a high density of negative charge is present on the surfaces of
the grafted microspheres. The strong electrostatic interaction results in which the grafted PMAA-HEMA/NVP
microspheres exhibit very strong adsoption ability toward lysozyme. The adsorption capacity of the grafted microspheres
for lysozyme initially increases and then decreases as the PH increases and a maximum adsorption(90 mg -)is found
at PH=9, which is close to the isoelectric point of l. The ionic strength affects the adsorption property of the
grafted microspheres greatly. As PH <9, the adsorption capacity of lysozyme decreases as the Nac1 concentration
increases, whereas as PH>9, the adsorption capacity of lysozyme increases as the Nacl concentration increases
Received: July 22, 2009; Rcvised: November 5, 2009; Published on Web: Deccmber 21, 2009
CorrespondingauthorEmail:gaobaojiao(@126.com;Tel:+86-351-3924795
The project was supported by the Natural Science Foundation of Shanxi Province, China(2008021013)
山西省自然科学基金(2008021013)资助项目
C Editorial office of Acta Physico-chimica Sinica