葡萄酒中赭曲霉毒素A检测方法优化.pdf

※分析检
2013.Wb.34.No.24193
葡蔔酒中赭曲霉毒羕A检测方法优化
庞世琦,刘青“,李志勇,潘丙珍,罗敏2,刘茜,陈文锐
(1.广东检验检疫技术中心,广东广州510623;2.南海出入境检验检疫局,广东佛山528200
摘要:对葡萄酒中描曲霉毒素A(OTA)的前处理和检测方法进行优化,分别采用液液萃取和免疫亲合柱前处理,
结合高效液相色谱-荧光检测(FPLC-FD)和醇联免疫法检测(LISA)对OTA的回收率、检测成本、检测效率及环保性
进行比较,逃出适用于大批量样品笳查的首选方法。同时针对目前大量进口的法国、西班牙、意大利、大利亚等
因家以及产的葡萄酒进行检测。结果表明:大批量葡萄酒样品的筛查建议采用液液萃取前处理结合EKSA方法进
行检测的组合作为首选,检测到阳性样品时再用免疫亲和柱净化后用高效液相色谱检泚进行确证,方法具有快捷、
灵敏、准确等特点,回收率、精密度都能达到检沨要求。
关縫词:赭曲毒素A;葡萄酒;高效液相色谐法;酶联免疫吸附测定法;方法优化
An Optimized Procedure for Rapid Determination of Ochratoxin A in Wine
PANG Shi-qi, LU Qing", LI Zhi-yong, PAN Bing-zhen LUO Min, LU Xi, CHEN Wen-rui
(1. Guangdong Inspection and Quarantine Technology Center, Guangzhou 510623, China
2. Nanhai Entry-exit Inspection and Quarantine Bureau, Foshan 528200, China)
Abstract: The purpose of this study was o stablish the optimal ample eatment prcedure and analytical method for
determining ochratoxin A(OTA)in wine. Samplepretreatment was achieved by alterative methods, either by liquid-liquid
ext raction r y immunaffi cl m chma raphy(A)and two i rent analytical methods, hih-performance
liquid chromatography (HPLC)and enzyme-linked immunosorbent assay(ELISA), were compared for their suitability
for large scale determination of OTA in wine from the iewoins of OTA recovery, cost, efficiency and environmental
protection. The selected anal tical procedure was validated by applying it for the ermination of wines imported from
France, Spain, Italy and Australia as well as domestic wnes Based on the results of this study, liquid-liquid extraction in
combinaion with ELSA is a more suitable method for large-scale screening of wines for TA and can be validated
comparative determination of the positive samples using HPLC analysis following AC clean-up. The method proposed in
this study is rapid, convement, sensitive and accurate and meets the analytical requirements of recovery and precision
Key words: ochratoxin A; wine; high p ormance liquid chromato raphy(HPLC enzyme-linked immuno soren
assay(ELSA); optimization
中图分类号:TS261.7
文献标志码:A
文章编号:1002-6630(2013)24-0193-04
doi:l0.75068pku10026630-2013240(0
赭曲霽毒素是曲菌属和青属的某些菌种产生癌症研究机构已将其定义为 group2B类致癌物冏。在葡萄
的二级有毒代谢产物,包含7种结构类似的化合物,其中酒的种植和隐制过程中,由于受种植环境和生产工艺、
赭曲霉毒燾A( ochratoxin A,OTA)在自然界中分布最广条件的影响,很容易造成赭曲霉毒素的污染。葡萄酒中
泛,毒性最强,对人类和动植物影响最大。OTA是一种OTA的检测越来越受到世界各国的重视
稳定的无色结晶化合物,具有耐热性,易溶于稀的碳酸
现有的OTA检测方法主要有液相色谱-荧光检测法、
氢钠溶液和极性有机溶剂,浴于水,在紫外照射下呈液相色谱-质谱联用法、酶联免疫法、毛细管电泳二极管
绿色荧光。研究表明,OTA不仅具有免疫毒性、肾毒性阵列检测法等。由于葡萄酒中的OTA含量较低(一般在
和肝毒性,并且还有致畸、致突变和致癌作用,图际0.1~5.pg~L),者虑到其成分复杂,可能会对目标检测
收稿日期:2012-12-02
基金项目:广东省科技计划项目(2011B05040005:2010B020316006):质检公益性行业科研专项(201210104-2):
广东检验检疫局科技计划项目(2013GDK31)
作者简介:庞世琦(1976-),女,工程师,学士,研究方向为食品安全与检测。E-mail:psqi@163.com
通信作者:刘青(1971),女,高级工程师,硕士,研究方向为食品安全与检测。E-mail:liuq@eiqtc.cn
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