环太湖水域微囊藻毒素-LR污染状况检测研究.pdf

第3卷第6期
食品安全质量检测学报
Vol 3 No 6
2012年12月
Journal of Food Safety and Quality
Dec.、2012
环太湖水域微囊藻毒素-LR污染状况
检测研究
张银志",李雪,周伟杰2,钮伟民2,刘萍2,晏丽
(1.江南大学食品科学与技术国家重点实验室,无锡214122
2.无锡市疾病预防控制中心,无锡214023)
摘要:目的了解环太湖沿岸主要城市生活饮用水中微囊藻毒素-工R的污染状况,建立环太湖水源水及无
锡、苏州和湖州自来水厂出厂水中MCLR浓度的时间分辦荧光免疫分析方法( TRFIA)。方法将偶联牛血清
白蛋白的MC-LR包被96孔板,密封抽真空后,加入MCLR标准溶液或样品,再加入抗MC-LR免血清后用全
自动 TRFIA检测仪测量,通过标准曲线计算样品中MC-LR的含量。结果环太湖水源水MC-LR检出率为
600%,平均浓度仅0.390pgL,自来水厂出厂水检出率为28.8%,其中全年检出最大量1.95gL。结论太湖
水存在藻毒素污染的问题,但是水质符合国家标准。
关键词:微囊藻毒素-LR;环太湖;时间分辨荧光免疫分析法( TRFIA)
Study on microcystins-lr pollution situation of Taihu rim
ZHANG Yin-zhi, LI Xue ZHOU Wei-jie, NIU Wei-min, LIU Ping, YAN
(1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China;
2. Wuxi Center for Disease Controland Prevention, Wuxi 214023, China)
ABSTRACT: Objective To investigate the drinking water pollution state of microcystins-lr(mc-lr)in
major cities around Tai lake and establish a method for the determination of MC-LR of source water and tap
water in the city of Wuxi, Suzhou and Huzhou around the Taihuby time-resolved fluorescence immunoassay
analysis(TRF]A). Methods The MC-LR coupled with bovine serum albumin was coated into the 96 wells
plate, vacuum sealed, added with MC-LR standard solutions or samplesand then was added with ANTI-MC-LR
bbit serum. After that, thecontent of MC-LR was detected through automatic TRFIA detector and calculated
by the standard curve. Results The concentration of MC-LR in source water in Tai lake was only 0.390 ug/L
in average, and 1.95 ug/L in maxium for the whole year, and the positive rate of source water and tap water
rere 60.0 and 28.8%, respectively. Conclusion The Tai lake water has MC-LR pollution problems, but it is
accorded with national standard
KEY WORDS: microcystins-lr; the Taihu lake rim; time-resolved fluorescence immunoassay analysis
基金项目:公益性行业(农业)科研专项(201003008)、无锡市科技局指导性项目(CS200958)
*通讯作者:张银志,高级工程师,研究方向:食品安全检测。Emai:yinzhizhangd@yahoo.com.cn
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