沉淀聚合法制备葛根素印迹微球及其固相萃取葛根粉.pdf

※工艺技术
品融字
2015. VOL36. NO10 II
沉淀聚合法制备葛根素印迹微球及其
固相萃取葛根粉
许苗苗,王素素,李辉2*,黄宇琳,逯翠梅,蒋剑波
(1.吉首大学化学化工学院,湖南吉首4160003
2.吉首大学植物资源保护与利用湖南省高校重点实验宰,湖南吉首416000)
摘要:以葛根素为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基内烯酸酯为交联剂沉淀聚合法备葛
根素印迹聚合物微球。用扫描电镜观察球形貌,静态吸附法测试聚合物的吸附行为。探讨分子印迹聚合物
( molecularly imprinted polymers,MIPs)对葛根粗提液中葛根素的吸附、解吸附及固相萃取效能。结果表明,优
化的葛根素MIP3对纯模板的静态吸附量达37.9mg/g:对粗提液中目标化合物的吸附率为(95.3±1.81)%,以水
甲醇-醋酸(9:1,I)及50%甲醇溶液为洗脱剂的单次解吸率为27%~34%,用水从MIP3上解吸出的总浴液经脱
除溶剂后,所获粗品葛根素含量最高,达(374士2.87)%。在优化条件下,通过MIP3固相萃取葛根粗提液,葛根
素回收率达71.6%,产品纯度高于75%
关犍词:分子印迹聚合物;葛根素:沉淀聚合法;吸附;固相萃取
Preparation of Puerarin-imprin ed Polymer Microspheres by Precipitation Polymerization Technique for
Use in Solid Phase Extraction of Flavonoids from Pueraria Root Powder
Xu Miaomiao WANG Susu! LI Huil2* HUANG Yulin LU Cuimei JLANG Jianbo
(1. College of Chemistry and Chemical Engineering, Jishou University, Jishou 416000, China,
2. Key Laboratory of Plant Resource Conservation and Utilization, Jishou University, Jishou 416000, China)
Abstract: Perarin-impind polymer microspheres(MPM were repared by rep ation pl m ization technique using
puerarin, methacrylic acid(MAA)and ethylene l ol dimh lacr lat(EGDMA)as the template, functional monomer and
cross-linker, respectively. Scanning electron micros oe(EM)was used for evaluating the morphology of polymer and a
static method was used for characterizing e a o on e a The aplication of the MIPM for adsorption, desorption
and solid phase extraction of puerain from crude pueraria root extract was explored. Results indicated that the adsorption
capacity for the optimized MIPM(M P3) toward the pure emplate reached 37.9 mg/g, with an adsorption percentage of
(96.3*1.81)% toward the target compound in the crude extract. The desorption percentage varied within the range of
27%4% when waer methanol-acetic acid(: an aquous methan were used as the elents, respectively
Furthermore, the product desorbed from the MIP3 using water as the eluent contained the highest content of puerarin
(37. 4#*2. 8% uea uc w h 5%uy can b one y MP3 ng n-s slid phase extraction with a
puerarin recovery of 71.6%
Key words: molecularly imprinted polymer; puerarin; precipi ation polymeization, adsorption; solid phase extraction
中图分类号:TS202.3
文献标志码:A
文章编号:1002-6630(2015)10-0011-05
dioi:10.7506/ spkxl002-6630-201510003
分子印迹聚合物( molecularly imprinted polymers,的结合孔穴,而对特定化合物具有选择识别能力。分子
MIPs)是在目标分子存在下,通过交联聚合制备而成的对印迹结合位点与模板分子间高选择、可逆、较高容量地
目标化合物具有记忆功能的高分子材料リ。因其基体中含相互作用,赋予印迹材料在分离分析及复杂样品选择萃
有与目标分子在大小、空间结构及化学基团等方面相匹配取方面的巨大优越性。迄今,MIPs已经广泛应用在色
收稿日期:2014-09-20
基金项目:国家自然科学基金面上项目(21077042):湖南省科技厅计划项目(2012SK3145)
作者简介:许苗苗(1987-),女,硕士研究生,研究方向为植物化学成分分离分析。E-mail:xumiaomiao2011@126.com
*通信作者:李辉(1968-),男,教授,博士,研究方向为生命科学中的分离分析新方法。E- mail: Lihuisdx (@163com
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