苦地胆内酯对嗜水气单胞菌感染斑马鱼保护效果的研究.pdf

第32卷第4期广东海洋大学学报Vol32 No42(012年8月Journal of Guangdong Ocean UniversityAug.2012苦地胆内酯对嗜水气单胞菌感染斑马鱼保护效果的研究宋春雨,聂芳红,林红英,刘実,马驿巨向红,陈进军,蔡吉榛,陆常青(广东海洋大学:1.农学院;2.食品科技学院广东湛江524088)摘要:在饲料中添加不同比例的苦地胆内酯,伺喂斑马鱼成鱼14d斤,采用创伤感染法构建斑马鱼嗜水单胞菌感染模型,研究苦地胆内对嗜水气单胞菌感染斑马鱼的保护效果。结果显示:饲喂含苦地胆内醋0.4%、0.8%、1.6%饲料的斑马鱼,其存活率显著高于阳性对照组(P值0.05);0.8%和1.6%6的苦地胆内对近马鱼的保护率分别为(73.91+6.52)%和(73.91+1130)%,与0.1%恩诺沙星保护率(76.08+7.53)%相比无显著差异(p值0.05)。一定剂量的苦地胆内脂对嗜水气单胞菌感染斑马鱼具有保护效果。关键词:苦地胆内酯;水气单胞菌;抗感染;斑马鱼中图分类号:S859.7;S948文献标志码文章编号:1673-9159(2012)04-008404Protective Effect of Elephantopus Scaber Lactones onZebrafish Infected by Aeromonas hydrophilaSONG Chun-yu, NTE Fang-hong, LIN Hong-ying LIU Han, MA YiJU Xiang-hong, CHEN Jin-jun, CAL Ji-zhen, LU Chang-qing(1. Agricultura/ College, Guangdong Ocean University; 2. ood Sci-fech College,(uangdong Ocean University, (guangdong, Zhanjiang 524088, ChinaAbstract: Diffcrcnt proportions of Elephantopus scaber lactones(ESL)were added in the dicts ofzebrafish. Each diet was used to feed adult fish for 1 4 days, and then wound infection method wasadopted to build Aeromonas hydrophila(AH infection model, Morbidity and mortality werrccordcd to study whcther ESL had in vivo anti-infcction cffcct against AH in zebrafish. The rcsultsshowed that fccd with 0. 4%, 0.8% and 1.6 of ESL significantly incrcascd the survival ratc of thczebralish Infected by AH 0.05): the protection rate of feed with 0.8%o and 1.6 of ESL were(73.91+6.52) and (73.91+11.30)%, respectively. And there were no significant differencesbctwccn the protcction ratc of fccd with 0.8% and 1.6% of ESL and thc protcction ratc of fecd with0. 1 enrofloxacin. All these results indicated that a cerlain dose of ESL has in vivo anli-infecuioneffect against AH in zebrafishKey words: Elephantopus scaber lactones, Aeromonas hydrophila, anti-infcction,Brachydanio rerio苦地胆( Elephantopus scaber Linr.)是一种抗菌中草药。地胆内酯(E, scaber lactones,分布广泛、来源丰富、有着深厚样众基础的传统ESL)作为其重要活性成分,对大肠埃希氏菌收稿日期:2012-05-18基金项目:广东省湛江市科技计划竞争性分配项日(洪科200694号)第一作者:宋春雨(1985-),男,碗士,主要从事中草药饲料添加剂的研究。通信作者:陈进车(1967-),博上,教授,研究方向为中药有效成分药效学与安全性评价。E-mail:jochen77yahoo.com.cn