高效液相色谱法测定丁醚脲在甘蓝和土壤中的残留及其消解动态分析.pdf

第8卷第6期
食品安全质量检测学报
Vol 8 No 6
2017年6月
Journal of Food Safety and Quality
Jun.,2017
高效液相色谱法测定丁瞇騄在甘蓝和士壤中的
残留及其消解动态分析
李増梅2,邓立刚,,赵善仓,2,郭长英2,董燕婕2,岳晖山
梁京芸2,赵艳3
(1.山东省农业科学院农业质量标准与检测技术研究所,济南250100,2.山东省食品质量与安全检测技术重点实验室,
济南250100;3.出东省泰安市畜收局,泰安271000)
摘要:目的建立高效液相色谱法检测甘蓝和土壤中丁脉的含量,并研究其在甘蓝和土壤中的代谢残
留。方法样品经乙腈高速匀浆提取,土壤采取振荡提取,旋转蒸发,浓缩,用乙腈定容,通过滤膜过滤,经
色谱柱分离。结果甘蓝、土壤中烯啶虫胺的最低检出浓度( limit of quantification,LOQ)均为0.01mgkg;在
0.02~100g/mL范围内丁醚脲的线性关系良好,R=10。甘蓝和土壤样品回收率在75.3%-102.3%,相对标准
偏差( relative standard deviation,RSD)为3.7%~1.4-%。代谢试验结果表明:2010年、2011年,河北、山东、
河南3地丁醚脲在甘蓝中的消解半衰期为1.2~2.8d,在土壤中的半衰期为1.8~4.4d;最终残留实验结果显示,
丁醚脲在甘蓝和土壤中的最终残留量均未检出,远低于最大残留限量( maximum residue limit,MRL)。结论在
自然条件下,丁醚脲属于易降解农药,在推荐使用剂量下,对收获后的甘蓝相对安全。
关键词:高效液相色谱法;丁醚脲;甘蓝;土壤;残留
Determination of residue of diafenthiuron and evaluation of its dynamics in
cabbages and soil by high performance liquid chromatograph
LI Zeng-meil2, DENG Li-gang 2, ZHAO Shan-cang'2, GUO Chang-ying, DONG Yan-jie
YUE Hui
1.2 LIANG Jing-yun
2、 ZHAO Yan
(1. Institute of Agricultural uality Standards and Testing Technology Research, Shandong Academy of Agricultura
Sciences, Jinan 250100, China: 2. Key Laboratory oftest Technology on Food Quality and Safety of Shandong
Province, Jinan 250100, China:; 3. Tai'an Animal Husbandry Bureaw of Shandong Province, Tai'an 271000, China)
ABSTRACT: Objective To establish a method for determination of diafenthiuron residue by high performance
liquid chromatography(HPL C)and evaluate the degradation dynamics of diafenthiuron in cabbage and soil. Methods
Samples were extracted by acetonitrile, and concentrated by rotary evaporation, then filtrated by 0.22
nembrane
The analytes were separated on a Waters Atlantis Ci8(2.1 mmx100 mm, 1.7 pm)and detected by HPLC. Results
The limits of quantification(LOQS)were both 0.01 mg/kg in cabbage and soil. The diafenthiuron had good linearities
in the range of 0.02-10 Hg/ML with correlation coefficient of 1.0. The recoveries of diafenthiuron in cabbage and sol
were 753-102.3%, and relative standard deviations (RSD )were 3.7-11.4%. The half-lives of diafenthiuron at
基金项目:山东省自然基金项目(ZR2015CM015
Fund: Supported by Shandong Natural Foundation(ZR2015CM015
*通讯作者:梁京芸,助理研究员,主要研究方向为食品质量与安全检測技术。E-mai:iyun@163.com
*Corresponding author: LIANG Jing-yun, Research Associate, Institute of Agricultural Quality Standards and Testing Technology Research
ShandongAcademyofagriculturalSciencesJinan251000,ChinaE-mail:Ijyun(@163.com
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