哈蟆油蛋白酶解物抗疲劳功效研究.pdf

食品研究与开发
2016年7月
基础研究
Food Research And Development
第37卷第13期
DOI:10.3969/.issn.1005-6521.2016.13.00
哈蟆油蛋白酶解物抗疲劳功效硏究
潘葉安,张鹤,董颖,王珊,边帅,王秋,齐滨,赵雨,赵大庆
(长春中医药大学,声林长春130117)
摘要:研究哈蟆油蛋白酶解物的抗疲劳作用。采用双酶法制备哈蟆油白酶解物,以哈蟆油蛋白晦解物低、中、高剂
量组和空白对照组连续给小鼠灌胃30d,测定小鼠负重游泳时间、血清尿素含量、血乳酸含量和肝糖原含壘。与空白对
照组相比,各剂量组小鼠负重游泳时间均明显延长(P0.05),高、中剂量组小鼠游泳后血清尿素含量显著降低(Pk0.05)
高剂量组小贔游泳后3个时间点血乳酸曲线下面积均小于空白对照组(Pk0.05);各剂量组小鼠肝糖原含量无显著性差
异(P-0.05)。哈蟆油蛋白酶解物具有缓解体力疲劳作用。
关键词:哈油;白酶解物;抗疲劳
Study on the Anti-fatigue Effect of Oviductus Ranae Protein Hydrolyzate
PAN Dai-an. ZHANG He. DONG Ying. WANG Shan. BIAN Shuai
WANG Qiu, QI Bin, ZHAO Yu, ZHAO Da-qing
Changchun University of Chinese Medicine, Changchun 130117, Jilin, China)
Abstract: Researching the anti-fatigue eflect of Oviductus Ranae protein hydrolyzate. Using double enzyme to
prepare Oviductus Ranae protein hydrolyzate. Four doses of Oviductus Ranae protein hydrolyzate with low
medium and high dose group and a blank control group were applied to mice continuously for 30 d Then mea
sured loaded swimming time, serum urea content, blood lactic acid content and hepalic glycogen of mice. Com
pared with blank control group, the loaded swimming time of mice of each dose group was significantly pro-
longed (P0.05), the serum urea content after swimming of mice of high and middle dose groups were both sig
nificantly lower (P0.05), the areas under the curve of the three time points of blood lactic acid ater swimming
of mice of high dose group were lower than the blank control group (P<0.05), the hepatic glycogen content of
each dose group was no significant differences(P>0.05). Oviductus Ranae protein hydrolyzate had the effect of
relieving physical fatigue
Key words: Oviductus Ranae; protein hydrolyzate; anti-fatigue
哈蟆油"( Oviductus Ranae)足中国林蛙( Rana tem-黄金”的美誉。现代药理学研究表明哈蟆油具有提高
poraria chensinensis David)雌蛙输卵管,采制后经干燥机体免疫力叫、抗氧化、抗波劳、抗应激等作用?。目前
而得,又称“林蛙油”,吉林省为主产区,是我国特有的对于哈蟆油成分的研究发现,主要成分为蛋白质,约
名贵中药材。哈蟆油味甘、成,性平,具有补肾益精、健占总量的50%以上网。有学者将哈蟆油分为水溶性成
脾益旹、滋阴补肾、润肺生津等功效,对体弱气虚、精力分和非水溶性成分,分别进行抗疲劳试验研究,研究
耗损、记忆力不佳、神经衰弱等疗效较好,富有滋补“软发现哈蟆油水溶性成分及其酶解物和非水溶性成分
及其晦解物都具有抗疲劳功效,且嗨解后抗波劳功效
进一步増强明。由于哈蟆油中的糖蛋白分子量大,黏
基金项日:吉林省医药产业发展引导资金项日(20150311031YY)
作者简介:潘黛安(1991ー),女(汉),倾土研究生,研究方向:中药有
度高,不易生产加工,采用質白酶酶解的方法可显著
效成分研究。
降低分子量,使其易于加工和人体消化吸收。但尚鲜
通信作者:张鶴(1985-),女,助理研究员:起大庆(1963-),男,有文献报道采用双海法制备得到的哈蟆油海解物的
教授。
药理活性,因此本实验采用蛋自酶和碱性蛋自酶双