磷酸香草醛法测定不同培养条件下四尾栅藻(Scenedesmus obliquus)总脂含量.pdf

CHINA OILS AND FATS
2017Vol.42No.7
生物工程
磷酸香草醛法测定不同培养条件下
四尾栅藻( Scenedesmus obliquus)总脂含量
张曼,郜小龙,张丽,祝伟霞
(1.河南师范大学水产学院,河南新乡453007:2.河南出入境检验检疫局,郑州450003
摘要:验证了磷酸香草醛反应在不同氮(0.2、2、20、40mg/L)、磷(0.1、1、10、20mg/L)质量浓度培
养条件下测定四尾栅藻总脂含量的可靠性。结果表明∷不同氮、磷质量浓度条件下栅藻总脂含量和
磷酸香草醛测定吸光度之间均呈显著的线性回归关系(不同氮质量浓度条件:R=0.997,P=
0.003;不同磷质量浓度条件:R=0.994,P=0.006),在不同氨、磷质量浓度培养条件下该方法的测
定结果准确可靠,可以用于栅藻的总脂含量测定。璘酸香草醛反应的显色强度可能与脂肪酸的饱
和度有关,为保障该方法测定总脂含量的准确性,用该方法估算总脂含量须在氮、磷质量浓度変化
范围內进行预先验证。
关键词微藻总脂含量磷酸香草醛反应測定方法
中图分类号:TS222:Q949文献标识码A
文章编号:1003-7969(2017)07-0120-04
Phosphoric acid -vanillin determination of total fat content of Scenedesmus
obliguus under different cultivation conditions
ZHANG Man. GAO Xiaolong ,ZHANG Li, ZHU Weixia
(1. College of Fisheries, Henan Normal University, Xinxiang 453007, Henan, China;
2. Henan Entry-exit Inspection and Quarantine Bureau, Zhengzhou 450003, China
Abstract The reliability of measuring total fat content of Scenedesmus obliquus using the phosphoric acid-vanillin
reaction under different nitrogen (0.2, 2, 20, 40 mg/L)and phosphorus (O 1, I, 10, 20 mg/L)mass concen-
tration cultural conditions was studied The results showed that there were sigmificantly linear regression relation-
ships between total fat content and absorbance value of phosphoric acid-vanillin determination under different ni-
trogen and phosphorus mass concentrations (different nitrogen mass concentration conditions: R=0.997, P
0.003 different phosphorus mass concentration conditions R=0.994, P=0.006). This method was accurate
and reliable under different phosphorus and nitrogen mass concentrations. It could be used to determine the total fat
content of Scenedesmus. The color intensity of phosphoric acid- vanillin reaction was associated with the saturation
degree of fatty acids. Therefore, the range of nitrogen and phosphorus mass coneent ration should be verified to en
sure the accuracy before using this method
Key words. microalgae, total fat content, phosphoric acid-vanillin reaction, determination method
受能源危机的影响,微藻在生产生物能源方面阳能转化为生物能,再通过不同的加工过程生产各
的潜力备受关注?。藻细胞能通过光合作用将太种生物燃料。与其他油料作物相比,微藻具有生长
速度快、产能高、油脂含量高、抗极端条件能力强等
收稿日期:2016-10-08:修回日期:2017-02-16
特点。微藻生产的油脂具有产量高、品性好、易
基金项目:国家自然科学基金(3140;河南省教育厅資于调控和改良等多重优势,目前对于微藻油脂的研
助项目(14B240004);河南师范大学博士启动金资助项目
究多集中在提高微藻含油量的方法上。但现今
()MD13(48)
作者简介:张曼(1982),女,副教授,博士,主要从事水域微藻生物燃料生产仍面临多重技术困难。其中,对
生态学研究工作(E-mail)2n0378@163.com
微藻粗脂肪含量的传统测定方法冗繁复杂,是微藻
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