解吸附电晕束电离质谱法快速检测微量雌激素.pdf

第4卷第3期
食品安全质量检测学报
VOL 4 No. 3
2013年6月
Journal of Food Salcly and Quality
um..2013
解吸附电晕束电离质谱法快速检冽微量雌激素
廖鹏,侯玉兰,吴婷婷,刘波,王华,陈波”
湖南师范大学,化学生物学及中药分析教育部重点实验室,长沙410081)
摘要:日的建立解吸附电晕束电离质谱(DCBI-MS)快速检測微量雌激素的分析方法。方法通过
DCBI-MS直接分析,对比标准物质一级和二级质谱图、对检測样品中非法添加的炔诺酮、黄体酮、甲羟孕酮、
左炔诺孕酮、醋酸甲地孕酮和栒櫞酸氯米芬等6种雌激素进行快速定性和半定量检测。结果各目标化合物
均可在1min内被检出。基于一级分子离子的定量分析,6种雌激素的半定量检测结果可信,线性关系最差的醋
酸甲地孕酮相关系数为0.9471,而黄体酮则为0.9998。最低检出值分别是0.0875、0.045、0.1775、0.05、0.15、
0.1mgL。结论本方法无需复杂的前处理过程,简便、快速、灵敏度高,可用于保健食品和中成药中违禁添
加微量雌激素的快速定性及半定量检测
关键词:解吸附电晕束电离质谱:雌激素;快速检测
Rapid determination of trace estrogens with DCBI-MS method
LIAO Pcng, HOU Yu-lan, WU Ting-ting, LIU Bo, WANG Hua, CHEN Bo
(Key aboratory for Chemica Biology c Traditiona Chinese Medicine Research, Ministry of ducation
Iluman Norma/ University, Changsha 410081, China)
ABSTRACT: Obiective A rapid method for detection of the trace estrogens was developed with the desorp-
Lion corona beam ionization mass spectrometry(dcbi-ms) technique. Methods The rapid qualilative and
semi-quantitative identification of 6 kinds of estrogens, including norethindrone, progesterone, medroxyproge
sterone acetate, levonorgestrel, megestrol acelate and clomiphene citrate in samples was perlormed by compar
ing MS and MS"spectra of samples with standard substances by DCBI-MS Results All of the 6 target mo-
lecules were delected within 1 min. Quantilicalion was achieved based on the molecular ion in MS spectra o!
cach analyte. The scmi-quantitative analysis for 6 estrogens was reliable. The lincar coefficient of megestrol
acctate was 0.9471 while progesterone was 0.9998. The LOD of 6 kinds of estrogens were 0.0875, 0.045
01775, 0.05, 0.15, 0.1 mg/L, respectively. Conclusion Without Tedious sample pretreatments, the developed
DCBI-MS is simple, rapid, sensitive for screcning and scmi-quantitative analysis of the estrogens additives in
health foods and traditional Chinesc patent medicines
KEY WORDS: desorption corona beam ionization mass spectrometry(dcbi-ms); estrogen: rapid detection
基金项目:国家自然科学基金项目(21275049)
Iund: Supported by the National Natural Science Foundation of China(21275049
通讯作者:陈波、教授,主要研究方向为分离分析化学,具体分析对象为食品和中草药。T- mail: dr- chenpo( 1p.sinae0)m
Corresponding author: HEN Bo, Professor, Kcy Laboratory for hcmical Biology &Traditional Chinesc Mcdicinc Rcscarch, Ministry of
Education, Hunan Normal Univcrsity, No 36, Lushan Road, Changsha 410081, China. E-mail: dr-chcnpo( vip. sina. com