苦丁冬青苦丁茶多糖的单糖组成分析.pdf

722013,Vo.34.No.11
品学
※基础研究
苦丁冬青苦丁茶多糖的单糖组成分析
范嘉龙,马丽苹2,叶红,曾晓雄,孙怡*
(1南京农业大学食品科技学院,江苏南京210095:2河南科技大学食品与生物工程学院,河南洛阳471003
摘要:对苦丁冬青苦丁茶多糖(IKPS)的分离纯化及其单糖组成进行研究。IKPS粗品通过 DEAE Sepharose Fast
FIow阴离子交换柱层析分离得到IKPS-1、IKPS-2、IKPS-3和IKPS-4共4个纯化组分,然后采用PMP柱前衍生
化法(HPLC法)和糖腈乙酸酯衍生化法(GC法)分析IKPS及其纯化组分的单糖组成。结果表明:IKPS、IKPS-1、
IKRS-2、IKPS-3和IKPS-4中均含有甘露糖、鼠李糖、半乳糖、阿拉伯糖、葡萄糖醛酸和果糖,除组分IKPS-4外
的其他组分均含有葡萄糖;另外,IKPS和IKPS-1中还含有岩藻糖,IKPS、IKPS-1和IKPS-4检测到了半乳糖醛
酸。2种方法检测结果基本一致,但HPLC法能检测到多糖组成中的果糖和糖醛酸信息,结果更为全面、可靠。
关键词:苦丁冬青苦丁茶;多糖;髙效液相色谱;柱前衍生化;气相色谱法
Monosaccharide Composition Analysis of Polysaccharides from Ilex audincha C J. Tseng I
FAN Jia-long', MA Li-ping, YE Hong, ZENG Xiao-xiong, SUN Yit
(1. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
2. College of Food and Bioengineering, Henan University of Science and Technology, Henan 471003, China)
Abstract: In the present study, crude polysaccharides from Ilex kudincha C.J. Tseng(TKPS)leaves were prepared
and purified by DEAE Sepharose Fast Flow anion-exchange column chromatography. As a result, four purified
fractions were obtained and named as IKPS-1, IKPS-2, IKPS-3 and IKPS-4. The monosaccharide composition of the
fractions was analyzed by two different methods: HPLC with pre-column derivatization with 1-pheny1-3-methyl-
5-pyrazolonde(PMP)and GC after derivatization to aldononitrile acetates. All these purified polysaccharides
were mainly composed of mannose, rhamnose, galactose, arabinose, glucuronic acid and fructose. Except IKPS-4
all other fractions contained glucose. Furthermore, IK.PS and IKPS-1 also contained fructose, and IKPS, IKPS-I
and IKPS-4 contained galacturonic acid. The results of HPLC analysis were generally consistent with those of
GC analysis. However, compared to GC analysis, HPLC analysis could provide more comprehensive and reliable
information about the monosaccharide composition of polysaccharides from Ilex kudincha C.J. Tseng leaves
Key words Ilex kudingcha C.J. Tseng; polysaccharides; high performance liquid chromatography; pre-column
derivaitzaiton; gas chromatography
中图分类号:Q946.3
文献标志码
文章编号:1002-6630(2013)11-0072-05
doi:10.7506/spkx1002-6630-201311017
苦丁茶冬青( ex kudincha CJ.Tse-ng)是冬青科冬青属冬青苦丁茶多糖提取工艺进行了优化,并研究发现苦丁
乔木植物,由其叶片加工而成的苦丁茶是我国南部和东冬青苦丁茶多糖对羟自由基、超氧阴离子自由基、DPH
部地区人民饮用的一种代用茶,苦丁茶也是传统中成药自由基具有一定的清除作用,对H2O2诱导的红细胞氧
“甘和茶”的主要原料之一。苦丁茶富含三萜及其皂苷化溶血反应和红细胞自氧化溶血反应都有显著的抑制作
类、多酚、氨基酸等活性物质,具有抗氧化、降脂、抗
用。何玲玲等采用95%乙醇脱脂、水提取及80%乙醇
炎杀薗、减肥、降血压等多种药理功能到。
沉淀的方法,从苦丁茶冬青叶中分离得到苦丁冬青苦丁
与茶叶多糖的研究相比“,有关苦丁冬青苦丁茶多茶粗多糖,经过特征化学反应及紫外光谱、红外光谱分
糖( nex kudincha C. Tseng polysaccharides,IKPS)的研析,确定了提取物为多糖类化合物,且含有一定量的蛋
究报道较少。吴晓鹏等7采用单因素和正交试验对苦丁白质。最近,Sun等对苦丁冬青苦丁茶多糖的提取、纯
收稿日期:2012-06-11
基金项目:中央高校基本科研业务费专项(KYZ201218);江苏高校优势学科建设工程资助项目
作者简介:范嘉龙1988-),女,硕土研究生,研究方向为食品生物技术。E-mil:2011049@njau.edu.cn
通信作者:孙佁(1966),女,讲师,博士,研究方向为食品营养与化学。E-mail:sunyol(@njau.edu.cn
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