高效液相色谱法测定八宝粥罐头中乙二胺四乙酸二钠含量的不确定度评定.pdf

粮食与食品工业
Vol.24,2017,No,3
Cereal and food Industrv
标准与检測
高效液相色谱法测定八宝粥罐头中乙二胺四
乙酸二钠含量的不确定度评定
刘四磊.赵允,戴绚丽,吴平
杭州娃哈哈集团有限公司(杭州310018)
摘要:根据JF1059.1-2012《测量不确定度评定与表示》的原理与方法,建立了高效液相色
谱法测定八宝粥罐头食品中乙二胺四乙酸二钠(EDTA-2Na)含量的測量不确定度评定的数学模
型。对数学模型中各不确定度分量的来源进行分析、量化和合成。当取样量为2.0g时,其测定的
乙二胺四乙酸二钠含量为(162.600士3.578)mg/L。最终结果的不确定度主要由标准溶液、样品
处理过程中回收率及标准曲线的建立引起。整体评定方法清晰合理,简便准确,可为实验室在该检
测过程中进一步提高检测数据的可靠性和一致性作参考。
关键词:乙二胺四乙酸二钠;不确定度;液相色谱;八宝粥罐头
中图分类号:TS297文献标识码:A文章编号:1672-5026(2017)03-065-06
Uncertainty evaluation for the determination of ethylenediamine tetraacetic acid
disodium saltin in canned eight-ingredient porridge by HPLC
Liu Silei, Zhao Yun, Dai Xuanli, Wu Ping
Hangzhou Wahaha Group Company Iimite (Hanguhou 10018
Abstract Based on JJF1050 1"Evalualion and E pression 0/ Uncertainly in Meas
urement, a mathematical model was established to assess the uncertainty of analyzing ethylene-
diamine tetraacetic acid disodium salt ( EITA-2NA) in canned eight-ingredient porridge b
high performance liquid chromatography (HPL C). Factors contributing to the uncertainty were
analyzed, quantified and synthesized. The expanded uncertainty of E1TA-2 Na determination
for 2.0 g of samples was 3. 578 mg/I. The results show that the uncertainty of measurement is
mainly caused by the preparation of standard solution, recovery of sample during processing and
the establishment of the standard curve. The evaluation method is reasonable, clear, simple, ac-
curate, and references for the reliability and consistency of the laboratory testing data
Key words: ethylenediamine tetraacetic acid disodium(ED"A 2 a); uncertainty; high per
formance liquid chromatography (HPLC); canned eight-ingredient porridge
乙二胺四乙酸二钠( EDTA-2Na)具有很高的由金属离子诱导产生的破坏食品感官性能的影
稳定常数,能够与绝大多数金属离子在微剂量下发响山。在GB27602014《食品添加剂使用标准》中
生络合反应形成稳定的化合物,能消除金属离子或规定其作为稳定剂、凝固剂、抗氧化剂、防腐剂,允许
使用于果酱,果脯、腌制蔬蔾、罐头、饮料等9类食品
收稿日期:2017-02-09
作者简介:刘四磊,男,1987年出生,硕士研究生,工程师,研
中。利用EDTA-2Na较强的络合作用,可有效
究方向为食品质量检测与安全控制
阻止或延缓食品褪色、氧化、变质,与防腐剂能表现
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