喂食家蚕核型多角体病毒诱导家蚕抗菌肽基因表达.pdf

南方农並学报 Journal of Southem Agriculture2015,46(12):2191-2195
ISSN 2095-1091: CODEN NNXAAB
http://www.nfnyxb.com
DO1:10.39695:is2095-1]191.2015.12.2191
喂食家蚕核型多角体病毒诱导家蚕
抗菌肽基因表达
唐芬芬2,杨伟克,张永红,朱峰,邵榆岚,白兴荣
云南省农业科学院蚕桑蜜蜂研究所,云南蒙自661101;2云南农业大学植物保护学院,昆明650201)
摘要:【目的】闻明家蚕抗菡肽基因对病毒感染的中肠免疫应答模式,为揭示昆虫抗病毒机制提供参考。[方
法】采用喂食家蚕核型多角体病毒( Bombyx mori nucleopolyhedrovirus, BMNPY)给家蚕的方式进行诱导,以实时荧
光定量PCR检测诱导后家蚕抗菌肱基?的表达情况,并比较 3MNPV诱?下8类已知家蚕抗菌肽主基因( cecropind
monti、 glowering2、 detension、 attacin I、 caborn2、 lysozyme和 lebocin3)在肠道组织中表达水平的差异。结果)在喂
食 BMNPV处理3时,家蚕中肠组织中旱上调表达的抗菌肽基因仅有 moricinj基因;处理6h时, Cecropind、 govering2
moricin基因转录水平均是明最的上謂趋势,尤其以 glowering2基因的诱导活性相对最高,是对照组 glowering2基因的
9.22倍;而在处理12和24b时检测,发现8类已知家蚕抗菌肽基因表达量均小于1.0,呈下调趋勢。【结论 BMNPV侵
染家蚕后 lovekin2基因表达量明显上调,可能是 lovekin2基因作为主要功能基因在病毒侵染早期过程中发挥了重
要作用
关键词:家蚕核型多角体病毒( BMNPV);抗菌肽基因;诱导;表达
中图分类号:S884.51
文献标志码:A
文章编号:2095-1191(2015)12-2191-05
Expression of antimicrobial peptide genes in Bombyx mori
induced by feeding B mori nucleopolyhedrovirus
TANG Fen-fen'-2, YANG Weike, ZHANG Yong-ho
SHAOYU-LAN, BAI Xing-rong/
g
('institute of Sericulture and Apiculture, Yunnan Academy of Agricultural Sciences, Mengzi, Yunnan 661101, China
2 College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China)
Abstract: [Objective The present experiment was conducted to investigate immune response patterns of antibacterial
de gencs to virus infetion in midgut, in order to provide references for antiviral mechanism of insect. [Method The
antibacterial peptide gones of Bombyx mori were induced by feeding on B. mori nucleopolyhedrovirus(BMNPV). Then the
sion of 8 kinds of major antimicrobial genes(cecropin, moricin, gloveri, defensin, atacind, en
lysozyme and ebocin 3)and their expression inferences were analyzed by real-time fluoresce l quantitative PCR ch
nique. [Result The results showed that, after feeding on EMNPY. the expressin of only ent mexican was up-regalaled al
3 h, and the transcription levels of ger cecropin gleverin2, tricin were sig ificantly increased a 6 h, especial
gene gloverin2 with higher induced transcriptional activity which was 9.2 times than that of control group. However, the
expression quantities of 8 kinds of antimicrobial genes in the midgut were less than 1.0 at 12 and 24 h, which showe
down-regulated trend. Conclusion Significant up-regulated expression of glovin2 in B. mor infected with BMNP
suggested that glavin gene can pay a role in the early stage of virus infection
Key words: B mori nucleopolyhedrovirus(BMNP ) antimicrobial peptide gene; induction; expression
0引言
进化过程中,已形成了抵御各种微生物侵染的第一道
研究意义昆虫在与做生物长期相互作用的共防线,即快速、多样、有效的先天免疫系统。其中,抗菌
收稿日期:2015-10-02
基金项目:国家自然科学基金项月(31260583);云南省现代农业蚕桑产业技术体系建设项目(2013KJ-TX(006)
作者简介:*为通讯作者,白兴菜(1974-),研究员,主要从事家蚕病理与分子生物学研究工作,E-mal:bxrong3@l63.coma,唐芬芬
(1985-),博士研究生,主要从事家蚕病理与分子生物学研究工作,E-mail:flavel985@163.com
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