铁氰化钾分光光度法测定头孢他啶.pdf

第24卷第10期
化学研究与应用
Vol 24.No 10
2012年10月
Chemical Research and Application
Oct,,2012
文章编号:1004-1656(2012)10-1540-04
铁氰化钾分光光度法测定头孢他啶
霍景娥,魏济时,李全民
河南师范大学化学与环境科学学院,河南省环境污染控制重点实验室,河南新乡453007)
摘要:建立了K[Fe(CN)6]光度法测定头孢他啶的方法。研究表明:控制溶液pH4.0左右,头孢他啶可以将
Fe(m)还原为Fe(m),还原生成的Fe(I)与K3[Fe(CN)。]反应生成可溶性的普鲁土蓝,其最大吸收波长为
735nm。头孢他啶在0.05-7.2g/mL范围内与吸光度呈良好的线性关系,线性回归方程A=0.03414+
0.07334c(g/mL),线性相关系数R=0.9992,表观摩尔吸光系数e=4.0x10(mol?cm),检出限为0.026
g/mL。本法能直接用于注射用头孢他啶的含量的测定,结果满意。
关键词:铁氰化钾;分光光度法;头孢他啶
中图分类号:0657.32文獻标识码:A
Spectrophotometric determination of ceftazidime with potassium ferricyanide
HUO Jing-e, WEI Ji-shi, L Quan-min
College of Chemistry and Environmental Science; Henan Normal University
Henan Key Laboratory for Environmental Pollution Control, Xinxiang 453007, China
Abstract: A novel method is established to determine ceftazidime by using potassium ferricyanide. Due to the reducibility of ceftazi-
dime, Fe(II[)is reduced to Fe (II )at PH 4. 0. Then Fe(II )reacts with potassium ferricyanide to form soluble prussian blue and its
maximal absorption wavelength is 735 nm. A god linear elation hip is obtained between the absor ance and the concentration of
ceftaz ime in he range of.0-7. m The equation of the linear regression is A=0. 03414+0.07334 (g /m), with a lin
ear correlat on coefficient of 999 The apparent molar absoption coefficient is 4. 0x/(mol em)and the detection limit is
0.026 g/ml The method is successfully applied to the determination of ceftazidime in in ection with satisfactory results
Key words: potassium ferricyanide; spectrophotometry; ceftazidime
头孢他啶( Ceftazidime)为半合成的第三代头钾可见光度法测定头孢他啶的方法。研究表明:
孢菌素,属于β-内酰胺类抗生素,对大多数革兰阳Fe(mI)被头孢他啶还原生成的Fe(I)与
性菌和阴性菌均有杀菌作用。目前,已报道测定K3[Fe(CN)5]反应生成可溶性的普鲁士蓝
头孢他啶的方法有:高效液相色譜法",紫外分光KFe"[Fe"(CN)。],通过测定 KFE [Fe"(CN)。]的
光度法?,流动注射化学发光法,悬汞电极阴极吸光度可以测定头孢他啶的含量。与上述方法相
溶出伏安法,毛细管区带电泳法?,荧光光度比,该方法具有操作简便、快速、分析成本低、不需
法?,可见分光光度法等。本文建立了铁氯化要使用较贵的仪器及设备等特点
收稿日期;2011-10-20;修何日期:2012-0405
甚金项目:河南省科技厅自然科学基金项目(082300420210)资助
联系人简介:李全民(1956-),男,教授,从事药物分析研究。E-mail:mercury6068@hotmail.com