马拉硫磷对双齿围沙蚕乙酰胆碱酯酶和过氧化氢酶的影响.pdf

农业坏境科学学报2010,29(3):431-436
Journal of Agro-environment Science
马拉硫磷对双齿围沙蚕乙酰胆碱酯酶和
过氧化氢酶的影响
吕林兰12,杨家新2,董学兴,黄金田1,吕
(1.盐城工学院化学与生物工程学院,江苏盐城224051;2.南京师范大学生命科学学院,南京210046)
摘要:采用浓度梯度污染暴露室内模拟方法,研究了沙蚕暴露于不同浓度有机憐农药马拉硫憐的急性毒性效应,以及低浓度马抗
硫磷对双齿围沙蚕乙酰胆破酯酶(AChE)和过氧化氡酶(CAT)的影响。结果表明,马拉硫磷对沙蚕48、72、96h的LC分别为71.68、
49.21、3.16mg-L,安全浓度为3.32mgL。在低浓度(0.0018-9mg-L)马拉硫磷中暴露3和6d,沙蚕体内乙酰胆碱酯酶活性受
到显著抑制,暴露6d时9mgL1浓度组最高抑制率达8892%,且乙酰胆碱詣酶抑制率与马拉硫磷浓度对数具有显著的剂量效应。
沙蚕CAT活性随马拉硫憐浓度増加表现出先降后升再降的趋势,暴露3d时0.018mg·L浓度组CAT活性最高,6d时0.18mg·L
浓度组CAT活性最高,分别比对照组高52.02%和53.42%(P<0.O5),而浓度达1.8mg?L时CAT活力均显著下降(P<0.05)。结果显
示,沙蚕乙酰胆碱酯酶是较好的滩涂有机磷农药污染生物标记物。
关键词:马拉硫礫;双齿围沙蚕;乙酰胆碱酶;过氧化氢酶
中图分类号:X503.223文献标志码:A文章编号:1672-2043(2010)03-0431-06
Effection of Malathion on te Activity of Acetycholinesterase and Catalase of Polychaete Perinereis aibuhitensis
IV Lin-lan 2, YANG Jia-xin, DONG Xue-xing, HUANG Jin-tian, LV Fu
( 1. College of Chemistry and Biology Engineering, Yancheng Institute of Technology, Yancheng 224051, China; 2. School of Iife Sciences
Nanjing Normal University, Nanjing 210046, China)
Abstract: Polychaete Perinereis aibuhitensis is considered to be a key species in estuarine ecosystem, and has proved to be a sentinel organ
ism for pollution stress. In order to test the possiblity of Perinereis aibuhitensis acting as an indicator organism to organophosphorus pesticide
malathion pollution, we conducted the experiment to determine the acute toxic effects of various concentrations of
aibuhitensis, and to examine the change pattern of their acetycholinesterase(ACHE )activity and catalase(AT)activity after exposed to low
concentration of malathion. The results indicated that the malathion had a strong toxic effect on P aibuhitensis. The 48 h, 72 h, 96 h L Cs val-
ues of P aibuhitensis were.,., 3.16 mg respe iel and e safety concentration wa 3.32 L. The ACHE actii of the
P bwh s was siany e e ma ho w h the maximum inhibition of.92% which appeared at ml on 6 d Mo
over the inhibition of ACHE significantly elated with the malathion concentration Malathion treatment led to an initial decline in CAT ac
tivity followed by a sharp increase and then declined again. These results showed that the ACHE activity of P. aibuhitensis could be used as a
good biomarkers to malathion pollution in coastal beach
Key words: malathion; polychaete Perinereis aibuhitens is; acelycholineslerase; catalase
我国自1983年起停止生产高毒、高残留、高富集机磷农药以杀虫剂居多(据统计,1997年生产有机磷
性的有机氯农药,而代之以低残留的有机农药。有杀虫剂约17万t,占整个农药使用量的50%左右),
使用后相当大一部分进入大气、地表水、地下水,形成
收稿日期:2009-08-06
跨介质污染,直接危害人类健康,且可以通过降水、地
基金项目:盐城工学院校级重点课题(XKY200732
表径流等方式流入海洋,对近海非目标生物造成严重
作者简介:吕林兰(1976一),女,重庆人,讲师,在读博士,主要从事水
域生态学和水产养殖教学与研究工作。
危害。而处于水-陆交错带的沿海滩涂是海洋生态系
i:lvlinlan@ycit.cn
统的重要组成部分,是鸟类和鱼、虾等生物的重要栖
万方数据