联合检测EB病毒不同抗体及EB病毒DNA在鼻咽癌血清学诊断中的价值.pdf

现代肿瘤医学2016年10月第24卷第19期 MODERN ONCOLOGY,Oc.2016,VOL.24,NO.19
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联合检测EB病毒不同抗体及EB病毒DNA在鼻咽癌血清学诊断中的
价值
叶倩,李筱莉,陈岩松,郭俊英,高嬌妮,余小龙,崔兆磊,陈燕
Diagnostic value of serological Epstein-barr viral anibodies and Epstein-barrviral DNA
assays in the management of nasopharyngeal carcinoma
Ye Qian, Li Xiaoli, Chen Yansong, Guojunying, Gao Yanni, Yu Xiaolong, Cui Zhaolei, Chen Yan
Department of Laboratory, Fuian Provincial Key Laboratory of Tumor Biotherapy, Fujian Medical University Teaching Hospital, Fuian
Provincial Cancer Ilospital, Fujian Fuzhou 350014, Chind
(Abstract Objective: To evaluate the diagnostic value of EB viral VCA-IGA, EA-IGA, Rta-igc and EB viral
DNA for nasopharyngeal carcinoma(NPC ) Methods: Serum and plasma samples were collected from 160 untreated
NPC patients, 133 NON-NPC patients with NPC-like symptoms and 163 healthy donors. EI.ISA was performed to te
tect VCA-IGA, EA-IGA and Rta -Igg antibodies in sera and Real-time fluorescent quantitative PCR was used for
measuring EBV DNA in plasma. According to the NPC 200 clinical stag ng T MI classification, different groups, the
clinical staging and before or after treatment of NP( that the difference of each antibody positive rate, antibody leve
and EB-D\A Lest results were analyzed. Results: The positive rates of VCA-IGA, EA-IGA, Rta-igg, EB\
DNA in NPC group were higher than those in nasopharyngeal related diseases and healthy control group(P<0. 05
Patienls in I Il slage yielded lower expression level(ra of EBVCA-IGA and EA-IGA than those in I, IV slage
P <0.05), but the positive rates revealed no statistically signifieant between these two groups(P>0.05).Addition
ally, EB Rta -Igg in patients with I, I stage harbored lower expression level(RA) and positive rates when com
pared to the cases in II, IV stage(P<0.05).Of note, the positive rate and the median level of EBV-DNA increasd
along with the clinical stages, and significant difference was observed between stage I, II and II I(P<0.05). Pa
tients with effective treatment(CR +PR displayed reduced FBV-D)NA level than that before treatment(P<
0.05).Conclusion VCA-IGA, EA-IGA, Rta-lgg, EBV-DNA can be utilized as useful biomarkers for NPC diag
nosis as well as the clinical stage prediction and therapeutic efficacy monitoring
Key words nasopharyngeal neoplasms, antibody, EB viral DNA, Real-lime fluorescent uanlitatie polymerase
chain reaction
Modern Oncology2016,24(19):3045-3048
【摘要」目的:评价EB病毒(EBV)抗衣売抗原VCA-1gA抗体、抗早期抗原EA-gA抗体、抗立即早期抗原
Rma-lgG抗体和FBV-DNA检测在鼻咽癖诊断中的价值。方法:收集160例初治鼻咽癌,133例症状相似的
非鼻咽癌患者和163名健康体检者的血清和血浆。采用酶联免疫法检测血清VCA-IgA、EA-IgA和Rna
lgC抗体的水平,用实时荧光定量PCR检测血浆EBV-DNA的相对含量。按鼻咽癌2008临床TNM分期法
收高H期)】2016-04-10
【修回日期
基金项目】福建省自然科学基金资助项目(编号:2014J01297)
【作者单位】福建医科大学教学医院,福建省肿生物治疗重点实验室,福建省肿瘤医院检验科,福建福州350014
【作者简介)叶情(1983-),女,福建宁德人.主管检验师,主要从事肿瘤实验诊断和临床免疫研究。E-mail:alyg@Isina.com
【通讯作者】陈應(1963-),女,福建福州人,主任医师,主要从事肿澶实验诊断的基础和临床研究。F-mnl:ranc99@sina.com