5,5-二乙烯基-2,2-联吡啶可能的新型锌离子荧光探针.pdf

第26卷第8期
无机化学学报
Vol 26 No8
2010年8月
CHINESE JOURNAL OF INORGANIC CHEMISTRY
I426-1430
5,5-二乙烯基-2,2-联吡啶:可能的新型锌离子荧光探针
韩焕美吴亚光肖守军
(南京大学配位化学国家重点实验室南京大学化学化工学院,南京210093)
摘要:生物体系里微量元素锌在发育、新陈代谢和疾病的发生等多个领域扮演了重要的角色,定性和定量测量锌的含量将帮助
我们理解锌的生物学意义。我们偶然发现5,5-二乙烯基-2,2-联吡啶(①VBP)在二氧六环里对锌离子呈现出类似比例计量型荧光
探针的特性:即有2个荧光发射峰而且长波长峰随锌离子的增加按比例増长。但DVBP难溶于水而且2个荧光发射峰距离太近
从而相互千扰,我们尝试利用硅氢反应将DVBP固定在多孔硅上制备感应锌离子的光极,我们发现多孔硅固相载体上的联呲啶
通过与锌离子的螯合后荧光增强约8倍,比DVBP在溶液里与锌离子螯合后的荧光增强(约4.5倍)还要多,联吡啶对锌离子的
荧光响应是一个值得继续探索的领域。
关键词:联吡啶;锌离子;荧光探针;多孔硅
中图分类号:0614.24+1;TO127.2
文献标识码:A
文章编号:1001-4861(201008-1426-05
5, 5-divinyl-2, 2-bipyridine: a Possible New Prototype of Fluorescent
Probe for Sensing Zn'
HAN Huan-mei WU Ya-guang XIAO Shou-jun
(State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering
fanjing University, Nanjing 210093)
Abstract: In biological systems, zinc-ion plays an important role in growth, metabolism, disease occurrence and
other aspects. Qualitative and quantitative measurements of zine-ion will help us understand the biological
significance of zinc-ion. We incidentally found thal 5, 5-diviny1-2, 2-bipyridinedvbp)in 1, 4-dioxane presented a
atiometric sensing feature toward Zn+, which possesses dual fluorescent emissions and the intensity of the long
wavelength emission increases ratiometricly with increasing the concentration of Zn *. However the non-solubility of
DVBP in aqueous media and its limitation for practical applications with the two emission bands overlapped severely
render us to search for alternative sensing approaches. We immobilized DVBP on porous silicon(PS)supporting
matrices for fabrication of Zn sensing optode. We found that Zn even enhanced the bipyridyl fluorescence 8
times, stronger than its enhancement of DVBP'S fluorescence(4. 5 times)in solution. Further research on fluorescent
response of bipyridine derivatives to Zn*should be continued
Key words: bipyridine; zinc ion; fluorescent probe; porous silicon
Fluorescence probes serve as useful tools in metal ion is of great interest in the field of neurobiology
biological investigations Zinc ion(Zn*)is one of the Fluorescent indicators that allow real-time imaging of
targets in the design of ratiometric probes, because this Zn* in living cells are invaluable tools for understan
收稿目期:2010-03-09。收修改稿日期:2010-04-09。
江苏省青蓝工程资助项目。
*通讯联系人。E-mail:suxiao(@nju.edu.cn
第一作者:韩焕美,女,27岁,博土研究生;研究方向:生物无机化学。