TNNT3突变转基因小鼠筛选及肌钙蛋白表达的研究.pdf

时伟红,等TNN3突变转基因小鼠筛选及肌钙蛋自表达的研究
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(编校:何妹)
INNT3突变转基因小鼠筛选及肌钙蛋白表达的研究
时伟红,李兆阳,杨,李翀,刘京津,郑志红
The screeningto study Of TNNT3 mutation transgenic mouse and the troponin TNNT3
(R69 H)gene expression of fast skeletal muscle in TNNT3 mutation transgenic mous
SHI Weihong, LI Zhaoyang, YANG Wei, LI Chong, LIU Jingjin, ZHGENG Zhihong
Department of Animal Laboratory, China Medical Uninersity, Liaoning Shenyang 110001, China
Abstract] Objective: To study the troponintnnt3(R69H )gene expression and tissue structure change of fast
skeletal muscle in TNNT3 mutation transgenic mouse. Methods: The heterozygotic TNNT3 mutation ransgenic
mouse was mated with same brood heterozygotic mouse. The positive progeny was detected by wild C57BL6.J mouse.
Fast skeletal muscle of wild mouse, heterozygotic TNNT3 mutation transgenic mouse and homozygous TNNT3 mutation
transgenie mouse were tested by to RT-PCR, WESTERN and electron microscope section. Results: The electropho-
resis band arrange of RT-PCR, WESTERN from bright to dark were homozygous mutation transgenic mouse, hetero
ygous mutation transgenic mouse, wild C57 BL/6J mouse. The electron microscope section of the fast skeletal muscle
of TNNT3 mutation transgenic mouse displayed band derangement, edema, vacuole. Conclusion: The quantity of
MRNA and protein of TNNT3 gene in homozygous mutation transgenic mouse is higher than heterozygous mutation
transgenic mouse, and heterozygous mutation transgenic mouse is higher than wild C57BL/6J mouse. The expression
of TNN T3(R69H )gene make structure abnormality of the fast skeletal muscle
Key words]TNNT3; transgene; mouse; troponin
Modern Oncology2012,20(02):0254-0256
摘要】目的:研究TNNr3(R69H)突变转基因小鼠快反应骨骼肌肌钙蛋白表达和组织结构的变化。方法
将杂合TNT3(R69H)突变转基因小鼠与野生型CS7BL/6J小鼠杂交,选同窝阳性杂合后代鼠进行交配,再用
野生型C57BL/6J小鼠对后代进行检测,得到TNTY3(R69H)突变转基因纯合个体。取野生型CS7BL/6J小
鼠、杂合转基因小鼠、纯合转基因小鼠的快反应骨骼肌进行RT-PCR和 WESTERN实验并制备透射电镜切
片。结果:RT-PCR和 WESTERN电泳条带从亮到暗依次为纯合转基因小鼠、杂合转基因小鼠、野生
57BL/6J小鼠。电镜结果显示转基因小鼠快反应骨骼肌明暗带不清、出现肌纤维间水肿和线粒体空泡等现
象。结论:TNT3(R69H)突变纯合转基因小鼠快反应骨骼肌中 TNNT3的mRNA和蛋白质量高于杂合转基因
小鼠、杂合转基因小風高于野生型C57BL/6J小鼠,并且转基因的表达使快反应骨骼肌结构异常
关键词) ITNNT3;转基因;小鼠;肌钙蛋白
【中图分类号】R73-74【文献标识码】ADOI1:10.3969/j1isn.1672-499.2012.02,11
【文章编号11672-4992-(2012)02-0254-03
【收稿日期】2011-07-20
修回日期】2011~-09-02
基金项目)辽宁省科学技术项目(编号:2009408001-3
【作者单位】中国医科大学实验动物学部,辽宁沈阳10001
【作者简介】时伟红(1961-),女,辽宁沈阳人,硕士,副教授,主要从事实验动物肿瘤干细胞研究。E-mail: swhmail@163,com