山薯组培苗继代培养基配方筛选.pdf

广西衷业科学2010,41(8):758-761
Guangxi Agricultural Sciences
山薯组培苗继代培养基配方筛选
严华兵1,龚明霞,董伟清,ト朝阳1,闭志强,李杨瑞
(1广西农业科学院a生物技术研究所;b虣莱研究所,南宁530007
摘要:以山翦组培苗带腋芽茎段为外植体,以MS为基本培养基,探讨植物生长调节剂6-BA(0.5、1.0、2.0mg/L)或
DZ(.01、0.10,0.50、1.00、2.00,400mgL)、不同培养方式和活性炭对其继代培养的影响。结果表明,添加不同浓度6
BA对山薯组培苗增殖系数、茎长及腋芽点芽球的影响不明显,而随着TDZ浓度的升高,山薯组培苗增殖系数和茎长逐
渐降低而芽球显著增加,茎段腋芽生长受到明显抑制。山薯组培苗较优的继代培养基为MS+1.0mgL6-BA+0.1mgL
NAA+30gL蔗糖+.5g/活性炭的液体培养基。液体培养基利于促进山薯组培苗增殖.其增殖系数、基长、植株鲜重和
干重晁著高于固体培养基。活性炭的添加可以减少褐化,促进组培苗的增殖培养。此外,培养方式与活性炭交互作用
对组培苗茎长、增殖系数、植株鲜重及干重无显著影响。
关键词:薯;继代培养;增殖系数;固体培养;液体培养;活性炭
中图分类号:S632.103.53
文献标识码:A
文章编号:1002-8161(2010)08-0758-04
Screening the subculture medium for Dioscorea fordii Prain et
Burk plantlets
YAN Hua-bing", GONG Ming-xia DONG Wei-qing, BU Zhao-yang
BI Zhi-qiang", LI Yang
(a Biotechnology Research Institute; b Vegetable Research Institute, 1 Guangxi Academy of Agricultural Sciences
Nanning 530007, China)
Abstract: The shoots with axillary buds derived from D fordii(Guihuai 2)plantlets were used as explants to investi
gate the effects of different plant growth regulators, viz., 6-BA(0.S, 1.0 and 2.0 m/ L and TDZ(0.01,0.10, 0.50 1.00, 2.00
and 4.00 mg/L)n MS medium, culture media( solid or iquid )and activated carbon (A ) on subculture of plantlets. The re-
sults showed that 1.0 mg/ 6-BA supplemented in MS basal medium had no si ificant effects on proliferation coeficient
stem length and gemmules derived from axillary buds. ith the increase in TDZ concentration in MS medium, the prolifer-
ation eoefficient, stem length of proliferated plantlets and growth of axillary buds inhibited significantly, while number
gemmule increased. The liquid MS medium +1.0 m/ L6-BA+1 m/L NAA+300 / L Sugar+lS g/L AC was found to be
the best medium for shoot! proliferation. Compared to semi-solid culture, the liquid culture promoted shoot proliferation sig
nificantly, proliferation coefficient stem length and fresh weight and dry weight of subculture plantlets were also found
higher. Addition of AC in MS medium reduced the browning of explants and promoted the shoot proliferation. Furt
ermo
the interaction between culture type and AC in MS medium had no significant effects on the proliferation coefficient, stem
dry weight of subculture plantle
Key words: Dioscorea ford Prain et Burk; subculture; proliferation coeficient; liquid culture; activated carbon
山薯( Dioscorea fordii Prain et Burk.),又称广行无性繁殖,采用自留种的栽培形式使其种性退
东准山,主要分布于广东、广西、福建、浙江南部和化,品质、产量严重下降。植物组织培养技术具有
湖南南部,生于海拔50-1150m的山坡、山坳、溪沟繁殖速度快、种苗可脱毒、不受季节限制等优点,其
边或路旁的杂木林中。山薯是薯蓣科( Dioscore-发展为植物的品种改良及离体快繁提供了一个重
aceae)薯蓣属( Dioscorea L.)一种药食兼用的高效要途径。目前,有关山薯的组织培养研究在国外还
经济作物,其块茎含丰富的淀粉以及其他人体必需未见报道,在国内仅有一篇研究报道。本研究以
的营养成分,具有重要的经济价值和特色产业开发“桂淮2号”优良山薯品种为研究对象,开展了山薯
潜力日。由于山薯长期以地上零余子或地下薯段进组培苗继代培养基配方筛选研究,为山薯良种繁育
收稿日期:2010-06-08
基金项目:广西科技攻关项目(桂科能06300桂科能0815011):广西农业科学院基本科研业务专项(200814基);广西大学博士
创新基金项目(200810593090101D020)
作者简介:严华兵(1979-),男,湖北孝感人,博士研究生,助理研究员,主要从事植物组织培养技术研究工作。
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