腐植酸影响扑草净对斑马鱼的急性毒性研究.pdf

2015,34(4):653-659
农业环境科学学报
2015年4月
Journal of Agro-ienvironment Science
腐植酸影响扑草浄对斑马鱼的急性毒性研究
赵倩,王灿灿,袁旭姣,朱琳
(南开大学环境科学与工程学院,污染过程与环境基准教育部重点实验室,天津市城市生态环境修复与污染防治重点实验室,天
津300071)
摘要:腐植酸(HA)是水环境中一类重要的溶解性有机质它能影响有机污染物对生物的急性毒性。以除草剂扑草浄( Prometryn)
作为口标污染物,模式生物斑马鱼( ao reto)作为受试生物,研究了扑草浄对斑马鱼的96h急性毒性以及HA存在下其对斑马鱼
的毒性变化情况。结果显示,扑草浄对斑马鱼的9%6h半致死浓度(96hLCq)为7.448mg·L,95%置信区间为(7.032~7.971)mg?L-。
5mg·1.1的HA对扑草浄的毒性没有显著影响,但当扑草净浓度为8.5mg?1.4时,15mg·.的HA增加了其对斑马色的9%h累积
死亡率,较同样扑草浄浓度下无IA组高出40%。研究表明在较高扑草净浓度下,15mg?LIA能增加其对斑马伯的急性毒性,这
可能是由」较高浓度(大」IC值)的扑草浄与HA共存时,减少污染物毒性的络合机制被其他能够引起毒性增加的机制所掩盖
关键词:腐植酸;扑草净;急性毒性;斑马鱼
中图分类号:X592文献标志码:A文章编号:1672-2043(2015)04-0653-07doi:10.11654/ae,2015.04.07
Influence of Humic Acid on Acute Toxicity of Prometryn to Danio rerio
ZHA0 Qian, WANG Can-can. YUAN Xu-jia0, /HU Iin
( Key I. aboralory of Pollulion Pro sses and Environmental Criteria. Tianjin Key Laboratory of Env ironmental Remediation and Pollulion Com
Lol, College of Euvironmeulal Seience and Engineering, Nankai University, Tianjin 300071, China
Abstract: Humic acid (HA is a principal component of dissolved organic matters in all aquatic ecosystems. It comprises of a variety of
molecular slruelures and functional groups and can bind pollutants in the waler, thereby affecting Ihe pollulanl loxicily lo aquatic organism
In Ihis sludy. a Ravine herbicide prometryn, commonly presen in the surface and ground waler was chosen as the largel pollutant and a
model organism zebrafish(Danio rerio )as the largel organism. The aim of this study was Lo investigate the aeule toxicity of prometryn to
Danio rerio at 5 and 15 mg L HA concentrations. Under single prometryn exposure. the 24 h 48 h. 72 h, and 96 h median lethal concen
trations (I C )of prometryn to Danio rerio were 9.143, 8.432, 7.645, and 7.448 mg L and 95 confience intervals were 8.635-9.86
7. 408-9.934.7.041-8.310. and 7.032-7.971 mg I. respectively. Based on these acute toxic experiment results, prometryn had the high
toxicity o Danio rerio. n addilion, 6 h cumulative morally rale of Dan rerio did nol change obviously with 5 mgl HA when the con-
eenlralions of prometryn were 6.5 7.5, and 8.5 mg. I.In conrast, e same toxicological parameter beeame 40%higher al 15 mg.1. HA
than Ha-free when prometryn concentration was 8.5 mg L. It was shown that HA can inerease the acute toxicity under higher prometryn
concentrations. This might be explained by a hypothesis that the association of prometryn with D)OM reducing its toxicity might be overshad
owed by other mechanisms actually enhancing its toxicity in water
Keywords: humic acid(HA): prometryn; acule loxoliey: zebrafish(anio rerio
三氮苯类除草剂是目前国内外广泛使用的选择类除草剂中常见的一种,主要通过抑制植物的电子
性内吸收传导型除卓剂,可防除一年生禾本科杂草传输来影响植物的光合作用,从而达到除?的作用。
和阔叶杂草及某些多年牛恶性杂草。扑草净(2-甲硫在水养殖业中也被频繁应用,主要用于清除鱼、
基-4,6-双异丙氨基均一氮术, Prometryn)是三.氮米虾、蟹、贝、海参等养殖水体中的丝状藻类(如浒)、大
型草类及其他有害藻类。扑草净在欧洲已于2004年
收稿日期:2014-11-18
被禁用,但由于它毒性低、杀草谱广,目前中国
基金项目:国家水体污染控制与治理不科技重大专(2012X0790-00美国、加拿大、新西兰、南非还在厂广泛使用
作者简介:赵情(1986-),女,河的柘城人,博士研究生,研究方向为
生态毒理学。F-mai: Italent(@163.om
扑草浄在环境中比较稳定,半衰期为13个月
通信作者:朱林E-mail:Zhulin@nankai.cdu.cn
其较长的残效期,更容易在环境中残并造成危害。