黄芪药材及其相关制剂的指纹图谱研究.pdf

第二军医大学
硕士学位论文
黄芪药材及其相关制剂的指纹图谱研究
姓名:陈婷婷
申请学位级别:硕士
专业:药物化学
指导教师:张卫东
20080401
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第二军医大学硕土学位论文
AST-7黄芪皂苷1
AST-8黄芪皂苷Ⅱ
AST-9黄芪皂苷mI
AST-10黄芪皂苷V
AST-11芒柄花素-7-O-β-D-毗喃葡萄糖苷AST-12毛蕊异黄酮-7-O-D-吡喃葡萄糖苷
关键词:黄芪,黄芪总皂苷,注射液,断血流皂苷A, HPLC/DAD,指纹图谱,相
似度
黄芪药材及其相关制剂的指纹图谱研究
Abstract
Radix Astragali, Huangqi in Chinese, the dried root of Astragalus membranaceus(Fisch)
Bge Var mongholicus (Bge. )Hsiao or A. membranaceus(Fisch)Bge, is one of the most
widely used hinese herbs prescribed in many Chinese formulas to reinforce Qi (vital
energy ) It was used for the treatment of nephritis, diabetes, hypertension, cirrhosis, cancer
etc. as an important tonic, an antiperspirant, an atonic, a diuretic and an adjunctive medicine
by Chinese doctors As Traditional Chinese Medicine(TCM) of tonic function, Radix
Astragali has been used thousands of years. The quality of TCM can be evaluated by the
hromatographic fingerprints, which represent the cmpound groups of TCM and substantial
foundation of pharmacodynamics. The chromatographic fingerprints can cater to the theory of
Chinese traditional medicines and characteristics of chemical constituents in TCM, thereby
also reflect the quality of TCM in an all-around way and be accepted by international. The
article established the chromatographic fingerprints of Radix astragali and preparations with
HILC/DAD
As reported previously, the chemical comprises of Radix astragali mainly includes
polysaccharides, flavonoids and saponins, among which saponins are commonly considered
as the major bioactive constituents. The fingerprints of Radix astragali, total Astragaloside
and its injection were investigated in this paper, so the saponins of Radix astragali were
inspected during the research course of fingerprint.
Firstly the pretreatment conditions of Radix astragali were inspected. By comparing
different solvent, extraction method and extraction time, the most optimized extraction
condition were ascertained with refluxing extraction with ethanol 30 mins. Then, the
chromatographic columns, detected wavelength and reference substance were investigated.
The UV absorption was large with stable baseline at 210nm. The Intersil ODS-3 column
was chosen as fingerprint analysis column with the wavelength at 210nm. Astragaloside V
was not suitable for reference substance because the peak of it was very small or not obvious.
Clinodiside A was ascertained to be reference substance and mixed by autosampler of the
HPLC instrument. The fingerprint analysis method of Radix Astragali from GAP producing
area was developed, and the methodology including precision, stability and reproducibility
were inspected. There were eight peaks in the chromatogram including clinodiside A The
fingerprints of the raw material of total Astragaloside and its injection were also developed