碲化镉-3-巯基丙酸(CdTe-3-MPA)量子点在大鼠四肢和胃部淋巴的显像.pdf

复?学报(医学版
Fudan Univ J Med Sci
2012Jul.,39(4)
碲化镉-3-巯基丙酸(CdTe-3-MPA)量子点在
大鼠四肢和胃部淋巴的显像
孙鹏'李鹏1?张旭瑞2张云鹛起
(复旦大学附属华山医院普外科上海200040:2复且大学高分子科学系上海20043
3复旦大学附属华山医院耳鼻喉头颂外科上海200040)
摘要】目的利用碲化镉-3-巯基丙酸(CdTe-3-MPA)核壳量子点在大鼠体内建立一种简单高效的淋巴实时显像
方法。方法分別于大鼠(れ=15)前肢皮下、后肢皮下、胃窦部胃小弯侧浆膜下注射量子点,观察量子点移行情况
结果四肢注射的量子点,15min即出现明显移行,前肢移行到胶窝,后肢则移行到腹股沟。随着时间的延长,量
子点继续沿着淋巴管移行,但始终没有越过前正中线。胃部注射的量子点,30min时移行至胃后面淋巴结。结论
CdTe-3-MPA核壳量子点的淋巴系统成功显像提示CdTe-3-MPA核売量子点具有辅助淋巴结显影的新潜力。
【关键词】量子点;淋巴结;影像学;大鼠
中图分类号】R73-37【文献标志码】A
doi:10.3969/j.issn.1672-8467.2012.04.010
Preliminary trials of CDT'E-3-MPA quantum dots in rats
extremities and stomach lymph node imaging
SUN Peng, LI Peng, ZHANG Xu-rui, ZHANG Yun-peng, ZHAO Xia
Department of General Surgery, Huashan Hospital,Fudan University, Shanghai 200040, Chine
partment of Macromol
Fudan University, Shanghai 200433, Ch
Department of Otolaryngology Head and Neck Surgery, Huashan Hospital, Fudan University, Shanghai 200040, China
Abstract Objective To develop a real-time imaging method for identifying lymph nodes with Cdte-
3-MPA quantum dots in rats. Methods Different rats (n=15) received CDTE-3-MPA quantum dots
injection at the subserosal layer in the lesser curvature of gastric antrums, the subcutaneous of anterior
and posterior limbs, respectively. Then draining lymphatic channels and lymph nodes were visualized b
a near-infrared fluorescence imaging system. Results Lymph nodes with CDTE-3-MPA quantum dots
were visualized after 15 minutes in the axillary fossa and groin after CDTE-3-MPA quantum dots
injection at the subcutaneous of anterior and posterior limbs. However, CDTE-3-MPA quantum dots did
not transfer to the contralateral parts in the whole process. Quantum dots were distributed in the back
of stomach 30 minutes after CDTE-3-MPA quantum dots injection at the stomach. Conclusions Near-
infrared fluorescence imaging of lymph nodes with CDTE-3-MPA quantum dots suggests a nove
potential for assisting images of lymph nodes
.ey words
quantum dots, lymph nodes; Imaging technique; rats
w This work was supported by the Science and Technology Commission Nano Special Fund of Shanghai Municipality
(1052nm03802)
上海市科委纳米专项基金项目(1052nm03802)
ACorrespondingauthorE-mail:lipengl129@live.cn