正交实验优化脾运康口服液总多糖提取工艺.pdf

第45卷第3期
应用化工
Vol 45 No. 3
2016年3月
Applied Chemical Industry
Mar.2016
正交实验优化脾运康口服液总多糖提取工艺
陈程',张存劳',贾渊2,冯锁民',陶鹏鹏
(1.西安医学院药学院,陕西西安710021;2.民航西安医院,陕西西安710082
摘要:用水煎煮法提取白术、杜蛎、蝉蜕、料姜石的总多糖,苯酚-硫酸法测定总多糖含量,考察加水量、煎煮时间
和醇沉浓度对多糖含量的影响。结果表明,最佳工艺为8倍量水,煎煮2次,每次60min,醇沉浓度50%。在此条
件下,提取液总多糖含量为12.88mg/mLo
关键词:脾运康口服液;制备工艺;总多糖
中图分类号:1TQ469;R283.61文献标识码:A文章编号:1671-3206(2016)03-0479-03
Optimization of preparation technology for total polysaccharide
from Piyunkang oral solution by orthogonal design
CHEN heng, ZHANG Cu-lao JA Yuan, FENG Suo-min TAO Peng-peng
(1. College of PI
arace
i 'an Medical College, Xi 'an 710021, China
2. Xi an Hospital of Civil Aviation, Xi 710082, China)
Abstract: The total polysaccharide in atractylodes, oyster, periostracum, liao-jiang stone were extracted by
water decocting. By phenolsulfuric acid method, the total polysaccharide content in the Piyunkang oral so
lution was determined. The effect of water addition, decocting time and alcohol precipitation concentration
on the total polysaccharide content were investigated. The result showed that the optimum technology was
adding 8-fold amount of water, extracted twice with 60 min for each time, and the alcohol concentration
was 50%. Under these condition, the total polysaccharide content were 12.88 mg/ml
Key words: Piyunkang oral solution; preparation technology; the total polysaccharide
儿童厌食症是儿科消化系统常见病,发病率为教师鉴定;D-无水葡萄糖对照品(中国食品药品检
12%~24%。中医认为,厌食症病在牌胃。脾为定研究院,批号110833201305);粮食酒精,食品
后天之本,脾失健运,冒不思纳,脾气通于口,脾不级;苯酚、浓硫酸均为分析纯;水为纯化水。
和,则不知五味2。脾运康口服液是在临床经验
UV-2012PSC型紫外可见分光光度计;
用方基础上开发的复方中药制剂,该制剂由白术、白Ac204电子天平;D2ZKW.D-2型水浴箱;LC32
蜂蜜、牡蛎、蝉蜕、料姜石为原料制备而成,具有扶正
低速离心机;KQ3200E型超声波清洗器。
固本、健脾助运的功效,用于小儿体虚厌食、消化不
良等症。为保证制剂质量,本文以水煎煮法提取方
1.2多糖提取
中白术、牡蛎、蝉蜕、料姜石的总多糖,采用苯酚-硫
按处方量称取白术、牡蛎、蝉蜕、料姜石,加水浸
酸法测定总多糖含量,通过单因素实验和正交实验泡,采用水煎煮法进行提取。提取液经过滤并浓缩
优化提取工艺,为进一步质量控制和开发提供理论至相对密度1.02~1.05(60℃)。水煎液中存在如
依据
树胶鞣质等杂质,在放置过程中会产生沉淀等现
实验部分
象,进而影响制剂稳定性,因此浓缩液加乙醇除去树
1.1试药与仪器
胶、鞣质等杂质,静置24h。取上清液,并回收乙醇
药材:白术牡蛎、蝉蜕、料姜石,由中药教研室加热水至100mL,待其室温,即得提取液。
收稿日期:2015-10-29修政稿日期:2015-12-04
基金项目:陕西省科技厅面上资助项目(2015JM8442);西安医学院学科建设经费资助
作者简介:陈程(1987-),男,陕西成阳人,西安医学院讲师,硕士,主要从事中药新技术应用研究。电话:13892033561,
E-mail:xayxychencheng@163.com
通讯联系人:张存劳(1960-),男,高级工程师。电话:18591921837,E-mail:1131168159@q.com